Wong Thomas C B, Rebbert Martha, Wang Chengdong, Chen Xiongfong, Heffer Alison, Zarelli Valeria E, Dawid Igor B, Zhao Hui
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P. R. China.
Int J Dev Biol. 2016;60(4-6):159-66. doi: 10.1387/ijdb.160058id.
Neural crest (NC) development is controlled precisely by a regulatory network with multiple signaling pathways and the involvement of many genes. The integration and coordination of these factors are still incompletely understood. Overexpression of Wnt3a and the BMP antagonist Chordin in animal cap cells from Xenopus blastulae induces a large number of NC specific genes. We previously suggested that Potassium Channel Tetramerization Domain containing 15 (Kctd15) regulates NC formation by affecting Wnt signaling and the activity of transcription factor AP-2. In order to advance understanding of the function of Kctd15 during NC development, we performed DNA microarray assays in explants injected with Wnt3a and Chordin, and identified genes that are affected by Kctd15 overexpression. Among the many genes identified, we chose Duf domain containing protein 1 (ddcp1), Platelet-Derived Growth Factor Receptor a (pdgfra), Complement factor properdin (cfp), Zinc Finger SWIM-Type Containing 5 (zswim5), and complement component 3 (C3) to examine their expression by whole mount in situ hybridization. Our work points to a possible role for Kctd15 in the regulation of NC formation and other steps in embryonic development.
神经嵴（NC）的发育由一个包含多种信号通路和众多基因参与的调控网络精确控制。这些因素的整合与协调仍未被完全理解。在非洲爪蟾囊胚的动物帽细胞中过表达Wnt3a和骨形态发生蛋白（BMP）拮抗剂脊索蛋白可诱导大量神经嵴特异性基因。我们之前曾提出，含钾通道四聚化结构域15（Kctd15）通过影响Wnt信号传导和转录因子AP - 2的活性来调节神经嵴的形成。为了进一步了解Kctd15在神经嵴发育过程中的功能，我们对注射了Wnt3a和脊索蛋白的外植体进行了DNA微阵列分析，并鉴定了受Kctd15过表达影响的基因。在鉴定出的众多基因中，我们选择了含Duf结构域蛋白1（ddcp1）、血小板衍生生长因子受体α（pdgfra）、补体因子备解素（cfp）、含锌指SWIM型结构域5（zswim5）和补体成分3（C3），通过全胚胎原位杂交来检测它们的表达。我们的工作表明Kctd15在神经嵴形成调控及胚胎发育的其他步骤中可能发挥作用。
