Onyeoziri Ukoha Pius, Romanus Ekere Nwachukwu, Onyekachukwu Uzodinma Irene
Department of Pure & Industrial Chemistry, Faculty of Physical Sciences, University of Nigeria, Nsukka, Nigeria.
Pak J Pharm Sci. 2016 Jul;29(4):1183-8.
This report assessed and compared the antioxidant potentials, quantities of ascorbic acid and phenolic compounds in methanolic extract of varieties of onions and garlic cultivars in Nigeria. The pH and total acidity of the extracts were equally determined. Antioxidancy of the cultivars were analysed using the in vitro assay techniques with 2,2-diphenyl-1-picryl Hydrazyl (DPPH) free radical scavenging and ferric reducing capacity. Ascorbic acid phenolic content were determined by volumetric and Folin-Ciocalteu's method respectively. The pH and total acidity were respectively 5.65 and 0.150mmol/L (red onion), 5.69 and 0.123mmol/L (white onion) and 6.94 and 0.105mmol/L (garlic). Red onion had the highest value of total phenols, ascorbic acid and free radical scavenging activity of 14.25±0.35mg GAE/ml, 229.098mg/100g, 66.44% respectively. In DPPH assay, red and white onion showed higher tendency to inhibit auto-oxidation when compared to garlic. The ferric reducing ability was greatest in garlic and least in white onions. These data indicate that with respect to antioxidant activity, red onion variety has highest health promoting potential among others.
本报告评估并比较了尼日利亚多种洋葱和大蒜品种甲醇提取物中的抗氧化潜力、抗坏血酸含量和酚类化合物含量。同时还测定了提取物的pH值和总酸度。采用体外分析技术,通过2,2-二苯基-1-苦基肼(DPPH)自由基清除法和铁还原能力分析法对各品种的抗氧化性进行了分析。分别采用容量法和福林-西奥尔特法测定抗坏血酸和酚类含量。红洋葱、白洋葱和大蒜提取物的pH值和总酸度分别为5.65和0.150mmol/L、5.69和0.123mmol/L、6.94和0.105mmol/L。红洋葱的总酚、抗坏血酸和自由基清除活性最高,分别为14.25±0.35mg GAE/ml、229.098mg/100g、66.44%。在DPPH分析中,与大蒜相比,红洋葱和白洋葱抑制自氧化的趋势更高。大蒜的铁还原能力最强,白洋葱最弱。这些数据表明,就抗氧化活性而言,红洋葱品种在其他品种中具有最高的促进健康潜力。