Tenyang N, Haman B Z, Tawai A J, Tabanty Z G, Douka G, Ponka R, Womeni H M
University of Maroua, Faculty of Science, Department of Biological Science, P.O. Box 814, Maroua, Cameroon.
University of Maroua, National Advanced School of Engineering, Department of Agriculture, Livestock and By-Products, P.O. Box 46, Maroua, Cameroon.
Heliyon. 2024 May 14;10(10):e31225. doi: 10.1016/j.heliyon.2024.e31225. eCollection 2024 May 30.
The present study investigated the antioxidant activities of methanolic extracts of two local plants and their capacities to preserve the quality of viscera fish oil during storage. The total phenolic, total flavonoids and tannins contents were analyzed by colorimetry, the preliminary antioxidant tests done and identification of phenolic compounds by HPLC-DAD (Diode Array Detector) and ESI-MS. After these evaluation, the plant extracts were added in viscera fish oil at concentration 200-1000 ppm. The oxidative stability of these fish oil samples was evaluated by measuring their qualities indices during 16 days at 70 °C. Here, oils were collected at the day 0, 4, 8, 12 and 16. The total phenolic, flavonoids and tannins contents in fruits were 15.62 g Eq acid galic/100g of dry extract, 10.85 mg Eq quercetin/100g of dry extract and 0.79 mg Eq cathechin/100 mg of dry extract. Those of leaves were 6.85 g Eq acid galic/100g of dry extract, 1.50 mg Eq quercetin/100g of dry extract and 0.11 mg Eq cathechin/100 mg of dry extract. All the methanolic extracts exhibited antioxidant activity. The results recorded after FRAP assay revealed the low IC50 (12.87 mg/mL) in extract compared to that of extract (44.90 mg/mL). These local plants contain many classes of phenolic compounds and they can be used in oil and fat industries as alternative of synthetic antioxidants.
本研究调查了两种当地植物甲醇提取物的抗氧化活性及其在储存期间保持内脏鱼油品质的能力。通过比色法分析总酚、总黄酮和单宁含量，进行初步抗氧化试验，并通过HPLC-DAD（二极管阵列检测器）和ESI-MS鉴定酚类化合物。经过这些评估后，将植物提取物以200 - 1000 ppm的浓度添加到内脏鱼油中。通过在70°C下测量16天内这些鱼油样品的质量指标来评估其氧化稳定性。在此，在第0、4、8、12和16天收集油样。果实中的总酚、黄酮和单宁含量分别为15.62 g当量没食子酸/100g干提取物、10.85 mg当量槲皮素/100g干提取物和0.79 mg当量儿茶素/100mg干提取物。叶子中的含量分别为6.85 g当量没食子酸/100g干提取物、1.50 mg当量槲皮素/100g干提取物和0.11 mg当量儿茶素/100mg干提取物。所有甲醇提取物均表现出抗氧化活性。FRAP试验后记录的结果显示，提取物的IC50较低（12.87 mg/mL），而提取物的IC50为（44.90 mg/mL）。这些当地植物含有多种酚类化合物，可在油脂工业中用作合成抗氧化剂的替代品。
