Eltony Sohair A
Histology Department, Faculty of Medicine, Assiut University, Egypt.
Tissue Cell. 2016 Aug;48(4):283-96. doi: 10.1016/j.tice.2016.06.009. Epub 2016 Jun 23.
Disorder in cerebellar structure was reported in diabetes mellitus. B vitamins are involved in many significant metabolic processes within the brain.
To detect the protective role of vitamin B complex on the histological structure of the cerebellum of experimentally induced diabetic rat.
MATERIAL & METHODS: Eighteen adult male Wistar rats were divided into two groups. Group I: normal vehicle control (n=6). Group II: streptozotocin-induced diabetic rats (n=12), which was equally divided into two subgroups; IIA (diabetic vehicle control), IIB (diabetic vitamin B complex-treated): streptozotocin-induced diabetic rats treated with vitamin B complex (1mg/kg/day) for 6 weeks. Specimens from the cerebellum were processed for light and electron microscopy.
In vitamin B complex treated group, the histological changes in Purkinje cells, astrocytes and oligodendrocytes were improved compared with the diabetic non-treated group. The white matter revealed intact myelinated axons. Inducible nitric oxide synthase (iNOS) and caspase-3 expression reduced. Glial fibrillary acidic protein (GFAP) expression revealed less activated astroglia. The number of Purkinje cells/mm(2) significantly increased. While, the number of GFAP positive astrocytes/mm(2) significantly decreased. In addition, the blood glucose level was reduced.
Vitamin B complex protected the cerebellum from the histological changes which occurred in STZ- induced diabetic rats.
