Shinohara Akihiko, Kameda Yuichi, Ibuki Shin-Ichi, Kiyoshi Takuya, Kakuda Tsuneo, Kojima Haruyoshi
Department of Zoology, National Museum of Nature and Science, 4-1-1 Amakubo, Tsukuba, Ibaraki, 305-0005 Japan.; Email:
Center for Molecular Biodiversity Research, National Museum of Nature and Science, 4-1-1 Amakubo, Tsukuba, Ibaraki, 305-0005 Japan.; Email:
Zootaxa. 2016 Apr 5;4098(1):167-78. doi: 10.11646/zootaxa.4098.1.8.
Based on field observations and rearing experiments in Nagano and Tochigi prefectures in central Honshu, Japan, host plants and life history of a leaf-rolling sawfly, Pamphilius ishikawai Shinohara, 1979, are recorded for the first time. The larva is a solitary leaf-roller on Astilbe. This is the first record of the Saxifragaceae as a host plant of the Pamphiliidae. We made molecular phylogenetic analysis using mitochondrial cytochrome oxidase subunit 1 (COI) gene sequences for nine individuals of P. ishikawai feeding on three different species of Astilbe in two localities with different climates and for six outgroup pamphiliine species. It revealed very small genetic variability within P. ishikawai and suggested probable monophyly of the P. sulphureipes group.
基于在日本本州中部的长野县和枥木县进行的实地观察和饲养实验，首次记录了卷叶锯蜂Pamphilius ishikawai Shinohara，1979的寄主植物和生活史。幼虫是落新妇上的单栖卷叶者。这是虎耳草科作为叶蜂科寄主植物的首次记录。我们利用线粒体细胞色素氧化酶亚基1（COI）基因序列，对在两个气候不同的地区取食三种不同落新妇的9只石川卷叶锯蜂个体以及6种外群叶蜂亚科物种进行了分子系统发育分析。结果显示，石川卷叶锯蜂内部的遗传变异非常小，并表明硫黄卷叶锯蜂组可能为单系群。
