Zha Tao, Liang Junrong, Xiao Yuchun, Jing Huaiqi
Bing Du Xue Bao. 2016 Mar;32(2):185-9.
To determine the lysis spectrum of Yersinia enterocolitica bacteriophage phiYe-F10 and to analyze the relationship between the lysis ability of phiYe-F10 and the virulence gene of Yersinia enterocolitica. To observe the lysis ability of the phage phiYe-F10 to the different Yersinia strains with the double-layer technique. The strains used in this study including 213 of Yersinia enterocolitica and 36 of Yersinia pseudotuberculosis and 1 of Yersinia pestis. The virulence genes of these Yersinia enterocolitica (attachment invasion locus (ail) and enterotoxin (ystA, ystB) and yersinia adhesin A (yadA), virulence factor (virF), specific gene for lipopolysaccharide O-side chain of serotype O : 3 (rfbc) were all detected. Among the 213 Yersinia enterocolitica, 84 strains were O : 3 serotype (78 strains with rfbc gene), 10 were serotype O : 5, 13 were serotype O : 8, 34 were serotype O : 9 and 72 were other serotypes. Of these, 77 were typical pathogenic Yersinia enterocolitica harboring with virulence plasmid (ail+, ystA+, ystB-, yadA+, virF+), and 15 were pathogenic bacterial strains deficiency virulence plasmid (ail+, ystA+, ystB-, yadA-, virF-) and the rest 121 were non pathogenic genotype strains. PhiYe-F10 lysed the 71 serotype O : 3 Yersinia enterocolitica strains which were all carried with rfbc+, including 52 pathogenic Yersinia enterocolitica, 19 nonpathogenic Y. enterocolitica. The phiYe-F10 can not lysed serotype O : 5, O : 9 and other serotype Y. enterocolitica, the lysis rate of serotype O : 3 was as high as 84.5%. The phiYe-F10 can not lysed Yersinia pseudotuberculosis and Yersinia pestis. Yersinia phage phiYe-F10 is highly specific for serotype O : 3 Yersinia enterocolitic at 25 degrees C, which showed a typical narrow lysis spectrum. Phage phiYe-F10 can lysed much more pathogenic Y. enterocolitica than nonpathogenic Y. enterocolitica.
测定小肠结肠炎耶尔森菌噬菌体phiYe-F10的裂解谱，并分析phiYe-F10的裂解能力与小肠结肠炎耶尔森菌毒力基因之间的关系。采用双层平板法观察噬菌体phiYe-F10对不同耶尔森菌菌株的裂解能力。本研究使用的菌株包括213株小肠结肠炎耶尔森菌、36株假结核耶尔森菌和1株鼠疫耶尔森菌。检测了这些小肠结肠炎耶尔森菌的毒力基因（黏附侵袭位点（ail）、肠毒素（ystA、ystB）和耶尔森菌黏附素A（yadA）、毒力因子（virF）、O:3血清型脂多糖O侧链特异性基因（rfbc））。在213株小肠结肠炎耶尔森菌中，84株为O:3血清型（78株有rfbc基因），10株为O:5血清型，13株为O:8血清型，34株为O:9血清型，72株为其他血清型。其中，77株为携带毒力质粒的典型致病性小肠结肠炎耶尔森菌（ail+、ystA+、ystB-、yadA+、virF+），15株为缺乏毒力质粒的致病菌株（ail+、ystA+、ystB-、yadA-、virF-），其余121株为非致病基因型菌株。PhiYe-F10裂解了71株均携带rfbc+的O:3血清型小肠结肠炎耶尔森菌菌株，其中包括52株致病性小肠结肠炎耶尔森菌、19株非致病性小肠结肠炎耶尔森菌。phiYe-F10不能裂解O:5、O:9血清型及其他血清型的小肠结肠炎耶尔森菌，O:3血清型的裂解率高达84.5%。phiYe-F10不能裂解假结核耶尔森菌和鼠疫耶尔森菌。小肠结肠炎耶尔森菌噬菌体phiYe-F10在25℃时对O:3血清型小肠结肠炎耶尔森菌具有高度特异性，显示出典型的窄裂解谱。噬菌体phiYe-F10裂解致病性小肠结肠炎耶尔森菌的能力比非致病性小肠结肠炎耶尔森菌更强。
