Petrova E S
Izv Akad Nauk Ser Biol. 2016 Mar-Apr(2):128-35.
Different subsets of interneurons in the Wistar rat neocortex and in neocortical transplants developing in a damaged nerve were identified by the following immunohistochemical markers: glutamate decarboxylase (GAD 67) for GABAergic nerve cells, NO-synthase (NOS) for NO-ergic neurons, choline acetyltransferase (ChAT) for cholinergic cells, and tyrosine hydroxylase for catecholaminergic structures. Twenty-eight days after surgery, individual GAD 67-ir, NO-ir, ChAT-ir, and very rarely TH-ir cells were detected in the graft. It was shown that the number of GAD 67-ir neurons per unit area in the grafts was less than in the rat neocortex P20.
通过以下免疫组化标记物鉴定了Wistar大鼠新皮层以及在受损神经中发育的新皮层移植体中不同的中间神经元亚群：用于γ-氨基丁酸能神经细胞的谷氨酸脱羧酶（GAD 67）、用于一氧化氮能神经元的一氧化氮合酶（NOS）、用于胆碱能细胞的胆碱乙酰转移酶（ChAT）以及用于儿茶酚胺能结构的酪氨酸羟化酶。手术后28天，在移植体中检测到了单个GAD 67免疫反应性、NO免疫反应性、ChAT免疫反应性以及极少的TH免疫反应性细胞。结果表明，移植体中每单位面积的GAD 67免疫反应性神经元数量少于P20大鼠新皮层中的数量。
