Bae Hong-Sook, Kim Hyun Ju, Jeong Da Hye, Hosoya Takahiro, Kumazawa Shigenori, Jun Mira, Kim Oh-Yoen, Kim Sun Wook, Ahn Mok-Ryeon
Nat Prod Commun. 2016 Apr;11(4):503-6.
Crowberry, Empetrum nigrum var. japonicum, is widely used in folk medicine and grows naturally in Korea. Although some constituents and biological activity of Korean crowberry have been examined, there is little detailed information available. In this study, we investigated the effects of ethanol extracts of crowberry (EECB) on the inhibition of angiogenesis, both in vitro and in vivo. The effects of EECB were tested on in vitro models of angiogenesis, that is, tube formation and proliferation of human umbilical vein endothelial cells (HUVECs). EECB exhibited significant inhibitory effects on tube formation of HUVECs in a concentration-dependent manner. In addition, crowberry significantly suppressed the proliferation of HUVECs in a concentration-dependent manner. Furthermore, strong antiangiogenic activity of EECB samples was observed in the in vivo assay using chick embryo chorioallantoic membrane (CAM). These results indicate that crowberry may have potential applications in the prevention and treatment of angiogenesis-dependent human diseases.
越橘，笃斯越橘变种日本笃斯越橘，在韩国民间医学中广泛使用且自然生长。尽管已对韩国越橘的一些成分和生物活性进行了研究，但详细信息却很少。在本研究中，我们调查了越橘乙醇提取物（EECB）在体外和体内对血管生成的抑制作用。EECB的作用在血管生成的体外模型中进行测试，即人脐静脉内皮细胞（HUVECs）的管形成和增殖。EECB对HUVECs的管形成表现出显著的浓度依赖性抑制作用。此外，越橘以浓度依赖性方式显著抑制HUVECs的增殖。此外，在使用鸡胚绒毛尿囊膜（CAM）的体内试验中观察到EECB样品具有很强的抗血管生成活性。这些结果表明，越橘在预防和治疗血管生成依赖性人类疾病方面可能具有潜在应用。
