Jermyn K A, Duffy K T, Williams J G
Imperial Cancer Research Fund, Clare Hall Laboratory, South Mimms, Hertfordshire, UK.
Nature. 1989 Jul 13;340(6229):144-6. doi: 10.1038/340144a0.
The characteristic structure of the mature Dictyostelium culminant is created by the regionalized cellular differentiation and directed movement of prestalk cells. The front prestalk zone of the migratory slug has previously been considered to be a homogeneous tissue. Here we demonstrate, however, the existence of multiple classes of prestalk cells located in different parts or the slug anterior. The pDd56 and pDd63 genes encoding closely related extracellular matrix proteins are dependent for their expression upon DIF-1, the specific stalk-cell inducer. We have fused the promoters of the two genes to a modified chloramphenicol acetyltransferase (cat) gene to produce immunologically detectable proteins which localize to the cell nucleus. These two markers define three distinct kinds of 'prestalk' cells. One class, which we term 'prestalk A' cells, expressed the pDd63 gene. 'Prestalk B' cells express pDd56 and may also express the pDd63 gene. A third class, which we term 'prestalk 0' cells, expresses neither marker.
成熟盘基网柄菌聚集丘的特征结构是由前柄细胞的区域化细胞分化和定向运动形成的。迁移期蛞蝓的前端前柄区以前被认为是一种均匀的组织。然而，我们在此证明，位于蛞蝓前部不同部位的前柄细胞存在多种类型。编码密切相关细胞外基质蛋白的pDd56和pDd63基因的表达依赖于特定的柄细胞诱导物DIF-1。我们已将这两个基因的启动子与一个修饰的氯霉素乙酰转移酶（cat）基因融合，以产生定位于细胞核的可通过免疫检测的蛋白质。这两个标记物定义了三种不同类型的“前柄”细胞。一类我们称为“前柄A”细胞，表达pDd63基因。“前柄B”细胞表达pDd56，也可能表达pDd63基因。第三类我们称为“前柄0”细胞，不表达这两种标记物。
