Pan Qiuling, Wang Ling, Dang Xiaoqun, Ma Zhengang, Zhang Xiaoyan, Chen Shiliang, Zhou Zeyang, Xu Jinshan
College of Life sciences, Chongqing Normal University, Chongqing, 401331, China.
Sericultural and Apicultural Institute, Yunnan Academy of Agricultural Sciences, Mengzi, Yunnan, 661101, China.
J Eukaryot Microbiol. 2017 Mar;64(2):278-281. doi: 10.1111/jeu.12346. Epub 2016 Jul 27.
The microsporidia Nosema bombycis is the insect pathogen of pebrine disease severely destructive to sericulture production. Here, we describe the use of Escherichia coli HT115 strain (DE3) to express double-strand RNAs targeting the gene encoding ADP/ATP protein in N. bombycis. The results showed that dsRNAs deferentially suppressed the gene expression during N. bombycis infection in the silkworm, and the effect waned gradually. Our results, for the first time, provide a tool to utilize the dsRNA expressed by recombinant E. coli to control the pebrine disease of the domestic silkworm.
微孢子虫家蚕微粒子虫是对养蚕生产具有严重破坏作用的微粒子病的昆虫病原体。在此，我们描述了利用大肠杆菌HT115菌株（DE3）表达靶向家蚕微粒子虫中ADP/ATP蛋白编码基因的双链RNA。结果表明，双链RNA在家蚕感染家蚕微粒子虫期间差异性地抑制了基因表达，且这种作用逐渐减弱。我们的结果首次提供了一种利用重组大肠杆菌表达的双链RNA来防治家蚕微粒子病的工具。
