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钾硼胁迫下棉花功能叶中物质组成的傅里叶变换红外光谱表征

[FTIR Spectroscopic Characterization of Material Composition in Functional Leaf of Cotton under Stress of Potassium and Boron].

作者信息

Wu Xiu-wen, Hao Yan-shu, Lei Jing, Jiang Cun-cang

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2016 Mar;36(3):676-80.

PMID:27400504
Abstract

Potassium (K) and boron (B) are essential nutrient elements for plants, and the elements play an important role for plant growth, development and physiological metabolism. Cotton has a higher demand for K and B; K deficiency or B deficiency often occurs in cotton though. To reveal the component changes in functional leaf of cotton under K and B stress and investigate effects on material composition from K and B. A pot experiment was conducted at Huazhong Agricultural University. (1) the characteristic peaks at 1 546.86, 1 438.85, 1 153.39 and 1 024.17 cm(-1) disappeared due to B deficiency, and relative absorbance of other characteristic peaks was decreased compared with normal, which suggested that the structures of protein, fiber, soluble sugar and ribosome in cotton functional leaf changed and decreased in cotent when lack of K. (2) the relative absorbance of all characteristic peaks was increased in the B-deficient cotton leaves compared with normal, suggesting B deficiency leads to the accumulation in leaves of protein, and fiber, soluble sugar and other carbohydrates because of the hindered transportation. (3) lack of both potassium and boron, induced significant changes to both the locations and relative absorbance of characteristic peaks, and the content of protein, and soluble sugar and other carbohydrates increased, while the content of nucleic acids and polysaccharides dropped. K deficiency led to the structures of protein, fiber, soluble sugar and ribosome in cotton functional leaf changed and decreased in content; B deficiency gave rise to the accumulation in leaves of protein, and fiber, soluble sugar and other carbohydrates; the content of protein and soluble sugar and other carbohydrates increased, while the content of nucleic acids and polysaccharides dropped when K and B were all in short supply.

摘要

钾(K)和硼(B)是植物必需的营养元素,这些元素对植物的生长、发育和生理代谢起着重要作用。棉花对钾和硼的需求量较高;然而,棉花中经常出现缺钾或缺硼的情况。为了揭示钾和硼胁迫下棉花功能叶的成分变化,并研究钾和硼对物质组成的影响。在华中农业大学进行了盆栽试验。(1)由于缺硼,1546.86、1438.85、1153.39和1024.17 cm-1处的特征峰消失,与正常情况相比,其他特征峰的相对吸光度降低,这表明棉花功能叶中蛋白质、纤维、可溶性糖和核糖体的结构发生变化,且缺钾时含量降低。(2)与正常情况相比,缺硼棉花叶片中所有特征峰的相对吸光度均增加,表明缺硼由于运输受阻导致蛋白质、纤维、可溶性糖和其他碳水化合物在叶片中积累。(3)钾硼都缺乏时,特征峰的位置和相对吸光度均发生显著变化,蛋白质、可溶性糖和其他碳水化合物的含量增加,而核酸和多糖的含量下降。缺钾导致棉花功能叶中蛋白质、纤维、可溶性糖和核糖体的结构发生变化且含量降低;缺硼导致蛋白质、纤维、可溶性糖和其他碳水化合物在叶片中积累;钾硼都缺乏时,蛋白质、可溶性糖和其他碳水化合物的含量增加,而核酸和多糖的含量下降。

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