Bochicchio Brigida, Bracalello Angelo, Pepe Antonietta
Laboratory of Protein-Inspired Materials, Department of Science, University of Basilicata, Potenza, Italy.
Chirality. 2016 Aug;28(8):606-11. doi: 10.1002/chir.22619. Epub 2016 Jul 12.
Materials inspired by natural proteins have a great appeal in tissue engineering for their biocompatibility and similarity to extracellular matrix (ECM). Chimeric polypeptides inspired by elastomeric proteins such as silk, elastin, and collagen are of outstanding interest in the field. A recombinant polypeptide constituted of three different blocks, each of them having sequences derived from elastin, resilin, and collagen proteins, was demonstrated to be a good candidate as biomaterial for its self-assembling characteristics and biocompatibility. Herein, taking advantage of the primary amine functionalities present in the linear polypeptide, we crosslinked it with 1,6-hexamethylene-diisocyanate (HMDI). The characterization of the obtained polypeptide was realized by CD spectroscopy, AFM, and SEM microscopies. The obtained results, although not conclusive, demonstrate that the crosslinked polypeptide gave rise to porous networks, thin nanowires, and films not observable for the linear polypeptide. Chirality 28:606-611, 2016. © 2016 Wiley Periodicals, Inc.
受天然蛋白质启发的材料因其生物相容性以及与细胞外基质（ECM）的相似性，在组织工程领域极具吸引力。受丝、弹性蛋白和胶原蛋白等弹性蛋白启发的嵌合多肽在该领域备受关注。一种由三个不同模块组成的重组多肽，每个模块都有源自弹性蛋白、 resilin和胶原蛋白的序列，因其自组装特性和生物相容性，被证明是一种很好的生物材料候选物。在此，利用线性多肽中存在的伯胺官能团，我们将其与1,6 - 六亚甲基二异氰酸酯（HMDI）交联。通过圆二色光谱（CD）、原子力显微镜（AFM）和扫描电子显微镜（SEM）对所得多肽进行了表征。所得结果虽然尚无定论，但表明交联多肽形成了线性多肽无法观察到的多孔网络、细纳米线和薄膜。手性28:606 - 611, 2016。© 2016威利期刊公司。
