Fujiwara Ryosuke, Noda Shuhei, Tanaka Tsutomu, Kondo Akihiko
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
Center for Sustainable Resource Science, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
J Biosci Bioeng. 2016 Dec;122(6):730-735. doi: 10.1016/j.jbiosc.2016.05.005. Epub 2016 Jul 9.
To produce styrene from a biomass-derived carbon source, Streptomyces lividans was adopted as a host strain. The gene encoding ferulic acid decarboxylase from Saccharomyces cerevisiae (FDC1) was introduced into S. lividans, and the resulting S. lividans transformant successfully expressed FDC1 and converted trans-cinnamic acid (CA) to styrene. A key factor in styrene production using microbes is the recovery of volatile styrene. In the present study, we selected polystyrene resin beads XRD-4 as the absorbent agent to recover styrene produced using S. lividans transformants, which enabled recovery of styrene from the culture broth. For styrene production from biomass-derived carbon sources, S. lividans/FDC1 was cultured together with S. lividans/p-encP, which we previously reported as a CA-producing S. lividans strain. This coculture system combined with the recovery of styrene using XAD-4 allowed the production of styrene from glucose, cellobiose, or xylo-oligosaccharide, respectively.
为了从生物质衍生的碳源生产苯乙烯，采用了变铅青链霉菌作为宿主菌株。将来自酿酒酵母的阿魏酸脱羧酶编码基因（FDC1）导入变铅青链霉菌，所得的变铅青链霉菌转化体成功表达FDC1并将反式肉桂酸（CA）转化为苯乙烯。利用微生物生产苯乙烯的一个关键因素是挥发性苯乙烯的回收。在本研究中，我们选择聚苯乙烯树脂珠XRD-4作为吸收剂，以回收使用变铅青链霉菌转化体产生的苯乙烯，这使得能够从培养液中回收苯乙烯。为了从生物质衍生的碳源生产苯乙烯，将变铅青链霉菌/FDC1与变铅青链霉菌/p-encP一起培养，我们之前报道变铅青链霉菌/p-encP是一种产生CA的变铅青链霉菌菌株。这种共培养系统与使用XAD-4回收苯乙烯相结合，分别允许从葡萄糖、纤维二糖或木寡糖生产苯乙烯。
