Amjadi Mohammad, Shokri Roghayeh, Hallaj Tooba
Department of Analytical Chemistry, University of Tabriz, Tabriz, East Azerbaijan, Iran.
Luminescence. 2017 May;32(3):292-297. doi: 10.1002/bio.3177. Epub 2016 Jul 13.
The interaction of glucose-derived carbon quantum dots (CQDs) with silver (Ag) and gold (Au) nanoparticles (NPs) was explored by fluorescence spectroscopy. Both metal NPs cause an efficient quenching of CQD fluorescence, which is likely due to the energy transfer process between CQDs as donors and metal NPs as acceptors. The Stern-Volmer plots were evaluated and corresponding quenching constants were found to be 1.9 × 10 and 2.2 × 10 M for AgNPs and AuNPs, respectively. The analytical applicability of these systems was demonstrated for turn-on fluorescence detection of the anti-cancer drug, 6-thioguanine. Because the CQD-AgNP system had much higher sensitivity than the CQD-AuNP system, we used it as a selective fluorescence probe in a turn-on assay of 6-thioguanine. Under optimum conditions, the calibration graph was linear from 0.03 to 1.0 μM with a detection limit of 0.01 μM. The developed method was applied to the analysis of human plasma samples with satisfactory results.
通过荧光光谱法研究了葡萄糖衍生的碳量子点（CQDs）与银（Ag）和金（Au）纳米颗粒（NPs）之间的相互作用。两种金属纳米颗粒均能有效猝灭CQD荧光，这可能是由于作为供体的CQDs与作为受体的金属纳米颗粒之间的能量转移过程所致。对Stern-Volmer曲线进行了评估，发现AgNPs和AuNPs的相应猝灭常数分别为1.9×10和2.2×10 M。这些体系的分析适用性通过对抗癌药物6-硫鸟嘌呤的荧光开启检测得到了验证。由于CQD-AgNP体系比CQD-AuNP体系具有更高的灵敏度，因此我们将其用作6-硫鸟嘌呤荧光开启检测中的选择性荧光探针。在最佳条件下，校准曲线在0.03至1.0 μM范围内呈线性，检测限为0.01 μM。所建立的方法应用于人体血浆样品的分析，结果令人满意。
