Banse Heidi E, Holbrook Todd C, Frank Nicholas, McFarlane Dianne
Department of Physiological Sciences (Banse, McFarlane) and Department of Veterinary Clinical Sciences (Holbrook), Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA; Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, Massachusetts 01536, USA (Frank).
Can J Vet Res. 2016 Jul;80(3):217-24.
Local (skeletal muscle and adipose) and systemic inflammation are implicated in the development of obesity-associated insulin resistance in humans. In horses, obesity is neither strongly nor consistently associated with systemic inflammation. The role of skeletal muscle inflammation in the development of insulin dysregulation (insulin resistance or hyperinsulinemia) remains to be determined. We hypothesized that skeletal muscle inflammation is related to obesity-associated hyperinsulinemia in horses. Thirty-five light-breed horses with body condition scores (BCSs) of 3/9 to 9/9 were studied, including 7 obese, normoinsulinemic (BCS ≥ 7, resting serum insulin < 30 μIU/mL) and 6 obese, hyperinsulinemic (resting serum insulin ≥ 30 μIU/mL) horses. Inflammatory biomarkers were evaluated in skeletal muscle biopsies and plasma. Relationships between markers of inflammation and BCS were evaluated. To assess the role of inflammation in obesity-associated hyperinsulinemia, markers of inflammation were compared among lean or ideal, normoinsulinemic (L-NI); obese, normoinsulinemic (O-NI); and obese, hyperinsulinemic (O-HI) horses. Skeletal muscle and plasma tumor necrosis factor alpha (TNFα) concentrations were negatively correlated with BCS. When comparing inflammatory markers among groups, skeletal muscle TNFα was lower in the O-HI group than in the O-NI or L-NI groups. In horses, neither skeletal muscle nor systemic inflammation appears to be positively related to obesity or obesity-associated hyperinsulinemia.
局部（骨骼肌和脂肪组织）及全身炎症与人类肥胖相关的胰岛素抵抗的发生有关。在马中，肥胖与全身炎症既没有强烈关联，也没有持续关联。骨骼肌炎症在胰岛素调节异常（胰岛素抵抗或高胰岛素血症）发生过程中的作用尚待确定。我们假设骨骼肌炎症与马的肥胖相关高胰岛素血症有关。对35匹身体状况评分（BCS）为3/9至9/9的轻型马进行了研究，其中包括7匹肥胖、正常胰岛素水平（BCS≥7，静息血清胰岛素<30 μIU/mL）的马和6匹肥胖、高胰岛素水平（静息血清胰岛素≥30 μIU/mL）的马。在骨骼肌活检组织和血浆中评估炎症生物标志物。评估炎症标志物与BCS之间的关系。为了评估炎症在肥胖相关高胰岛素血症中的作用，比较了瘦型或理想型、正常胰岛素水平（L-NI）；肥胖、正常胰岛素水平（O-NI）；以及肥胖、高胰岛素水平（O-HI）马之间的炎症标志物。骨骼肌和血浆肿瘤坏死因子α（TNFα）浓度与BCS呈负相关。在比较各组的炎症标志物时，O-HI组的骨骼肌TNFα低于O-NI组或L-NI组。在马中，骨骼肌炎症和全身炎症似乎均与肥胖或肥胖相关高胰岛素血症无正相关。
