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本文引用的文献

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Are biological agents toxic to human chondrocytes and osteocytes?生物制剂对人类软骨细胞和骨细胞有毒性吗？
J Orthop Surg Res. 2015 Jul 30;10:118. doi: 10.1186/s13018-015-0264-y.
2
Clonal culture of adult mouse lung epithelial stem/progenitor cells.成年小鼠肺上皮干细胞/祖细胞的克隆培养
Methods Mol Biol. 2015;1235:231-41. doi: 10.1007/978-1-4939-1785-3_17.
3
Isolation and characterization of human intestinal mast cells.人肠道肥大细胞的分离与鉴定
Methods Mol Biol. 2015;1220:163-77. doi: 10.1007/978-1-4939-1568-2_11.
4
Evaluation of novel in situ synthesized nano-hydroxyapatite/collagen/alginate hydrogels for osteochondral tissue engineering.用于骨软骨组织工程的新型原位合成纳米羟基磷灰石/胶原蛋白/海藻酸盐水凝胶的评估
Biomed Mater. 2014 Oct 30;9(6):065004. doi: 10.1088/1748-6041/9/6/065004.
5
Strategies to improve homing of mesenchymal stem cells for greater efficacy in stem cell therapy.提高间充质干细胞归巢能力以增强干细胞治疗效果的策略。
Cell Biol Int. 2015 Jan;39(1):23-34. doi: 10.1002/cbin.10378. Epub 2014 Oct 13.
6
Does insulin, transferrin and selenous acid preparation effect chondrocyte proliferation?胰岛素、转铁蛋白和亚硒酸制剂对软骨细胞增殖有影响吗？
Acta Orthop Traumatol Turc. 2014;48(3):313-9. doi: 10.3944/AOTT.2014.2635.
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Regulation of chondrogenesis by protein kinase C: Emerging new roles in calcium signalling.蛋白激酶C对软骨形成的调控：钙信号传导中的新作用初现
Cell Signal. 2014 May;26(5):979-1000. doi: 10.1016/j.cellsig.2014.01.011. Epub 2014 Jan 17.
8
Gene expression during induced differentiation of sheep bone marrow mesenchymal stem cells into osteoblasts.绵羊骨髓间充质干细胞诱导分化为成骨细胞过程中的基因表达
Genet Mol Res. 2013 Dec 11;12(4):6527-34. doi: 10.4238/2013.December.11.4.
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C/EBP homologous protein drives pro-catabolic responses in chondrocytes.C/EBP 同源蛋白驱动软骨细胞中的促分解代谢反应。
Arthritis Res Ther. 2013;15(6):R218. doi: 10.1186/ar4415.
10
How different methodologies of harvesting and analysing the samples affect the test results in determining joint mediators.采集和分析样本的不同方法如何影响在确定关节介质时的测试结果。
Arthritis. 2013;2013:631959. doi: 10.1155/2013/631959. Epub 2013 Feb 20.

一种制备原代人软骨细胞培养物的实用方法。

A practical way to prepare primer human chondrocyte culture.

作者信息

Isyar Mehmet, Yilmaz Ibrahim, Yasar Sirin Duygu, Yalcin Sercan, Guler Olcay, Mahirogullari Mahir

机构信息

Istanbul Medipol University School of Medicine, Department of Orthopaedic and Traumatology, 34214 Istanbul, Turkey.

Republic of Turkey, Ministry of Health, State Hospital, Department of Pharmacovigilance and Rational Drug Use Team, 59100 Tekirdag, Turkey.

出版信息

J Orthop. 2016 Apr 1;13(3):162-7. doi: 10.1016/j.jor.2016.03.008. eCollection 2016 Sep.

DOI:10.1016/j.jor.2016.03.008
PMID:27408489
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4919283/
Abstract

Biological cartilage repair is one of the most important targets for orthopedic surgeons currently. For this purpose, it is mandatory to know how to prepare a chondrocyte culture. In this study, our purpose was to introduce a method enabling orthopedic surgeons to practice their knowledge and skills on molecular experimental setup at cellular level, based on our experiences from previous pilot studies. Thus, we believe it will encourage orthopedic surgeons.

摘要

生物软骨修复是目前骨科医生最重要的目标之一。为此，必须知道如何制备软骨细胞培养物。在本研究中，我们的目的是基于我们之前的初步研究经验，介绍一种方法，使骨科医生能够在细胞水平的分子实验设置上实践他们的知识和技能。因此，我们相信这将鼓励骨科医生。