Zhao Guangyu, Duan Zheng, Ming Lian, Li Yiyun, Chen Ruipeng, Hu Jiandong, Svanberg Sune, Han Yanlai
Appl Opt. 2016 Jul 1;55(19):5273-9. doi: 10.1364/AO.55.005273.
Laser-induced fluorescence is an important technique to study photosynthesis and plants. Information on chlorophyll and other pigments can be obtained. We have been using a mobile laboratory in a Chinese experimental farm setting to study maize (Zea mays L.) leaves by reflectance and fluorescence measurements and correlated the spectroscopic signals to the amount of fertilizer supplied. Further, we studied five different species of maize using the remote monitoring of the fluorescence signatures obtained with the same mobile laboratory, but now in a laser radar remote-sensing configuration. The system separation from the target area was 50 m, and 355 nm pulsed excitation using the frequency-tripled output from an Nd:YAG laser was employed. Principal component analysis and linear discriminant analysis were combined to identify the different maize species using their fluorescence spectra. Likewise, the spectral signatures in reflectance and fluorescence frequently allowed us to separate different fertilizer levels applied to plants of the same species.
激光诱导荧光是研究光合作用和植物的一项重要技术。可以获取有关叶绿素和其他色素的信息。我们一直在中国的一个实验农场环境中使用移动实验室，通过反射率和荧光测量来研究玉米（Zea mays L.）叶片，并将光谱信号与肥料施用量相关联。此外，我们使用同一移动实验室获得的荧光特征进行远程监测，研究了五个不同品种的玉米，但现在是在激光雷达遥感配置下进行的。系统与目标区域的距离为50米，采用了利用Nd:YAG激光器三倍频输出的355纳米脉冲激发。主成分分析和线性判别分析相结合，利用荧光光谱识别不同的玉米品种。同样，反射率和荧光中的光谱特征常常使我们能够区分施用于同一品种植物的不同肥料水平。
