Steiner Florian, Hauser-Kronberger Cornelia, Rendl Gundula, Rodrigues Margarida, Pirich Christian
Department of Pathology, Paracelsus Medical University Salzburg, Müllner Hauptstrasse 48, A-5020 Salzburg, Austria.
Department of Nuclear Medicine and Endocrinology, Paracelsus Medical University Salzburg, Müllner Hauptstrasse 48, A-5020 Salzburg, Austria.
Int J Mol Sci. 2016 Jul 9;17(7):1093. doi: 10.3390/ijms17071093.
Tenascin C expression correlates with tumor grade and indicates worse prognosis in several tumors. Epidermal growth factor receptor (EGFR) plays an important role in driving proliferation in many tumors. Loss of E-cadherin function is associated with tumor invasion and metastasis. Thyroid transcription factor-1 (TTF-1) is involved in rearranged during transfection (RET) transcription in Hirschsprung's disease. Tenascin C, EGFR, E-cadherin, TTF-1-expression, and their correlations with RET mutation status were investigated in 30 patients with medullary thyroid carcinoma (MTC) (n = 26) or C-cell hyperplasia (n = 4). Tenascin C was found in all, EGFR in 4/26, E-cadherin in 23/26, and TTF-1 in 25/26 MTC. Tenascin C correlated significantly with tumor proliferation (overall, r = 0.61, p < 0.005; RET-mutated, r = 0.81, p < 0.01). E-cadherin showed weak correlation, whereas EGFR and TTF-1 showed no significant correlation with tumor proliferation. EGFR, E-cadherin, and TTF-1 showed weak correlation with proliferation of RET-mutated tumors. Correlation between TTF-1 and tenascin C, E-cadherin, and EGFR was r = -0.10, 0.37, and 0.21, respectively. In conclusion, MTC express tenascin C, E-cadherin, and TTF-1. Tenascin C correlates significantly with tumor proliferation, especially in RET-mutated tumors. EGFR is low, and tumors expressing EGFR do not exhibit higher proliferation. TTF-1 does not correlate with RET mutation status and has a weak correlation with tenascin C, E-cadherin, and EGFR expression.
肌腱蛋白C的表达与肿瘤分级相关，且在多种肿瘤中提示预后较差。表皮生长因子受体（EGFR）在许多肿瘤的增殖过程中起重要作用。E-钙黏蛋白功能缺失与肿瘤侵袭和转移相关。甲状腺转录因子-1（TTF-1）参与先天性巨结肠病中转染重排（RET）转录过程。对30例甲状腺髓样癌（MTC）患者（n = 26）或C细胞增生患者（n = 4）的肌腱蛋白C、EGFR、E-钙黏蛋白、TTF-1表达及其与RET突变状态的相关性进行了研究。在所有MTC中均发现有肌腱蛋白C，4/26例中有EGFR，23/26例中有E-钙黏蛋白，25/26例中有TTF-1。肌腱蛋白C与肿瘤增殖显著相关（总体，r = 0.61，p < 0.005；RET突变型，r = 0.81，p < 0.01）。E-钙黏蛋白显示出弱相关性，而EGFR和TTF-1与肿瘤增殖无显著相关性。EGFR、E-钙黏蛋白和TTF-1与RET突变型肿瘤的增殖呈弱相关。TTF-1与肌腱蛋白C、E-钙黏蛋白和EGFR的相关性分别为r = -0.10、0.37和0.21。总之，MTC表达肌腱蛋白C、E-钙黏蛋白和TTF-1。肌腱蛋白C与肿瘤增殖显著相关，尤其是在RET突变型肿瘤中。EGFR表达较低，表达EGFR的肿瘤未表现出更高的增殖。TTF-1与RET突变状态无关，与肌腱蛋白C、E-钙黏蛋白和EGFR表达呈弱相关。
