Puzach S S, Gorbach Z V
Vopr Med Khim. 1989 Mar-Apr;35(2):82-4.
Tiaminase I (EC 2.5.1.2) catalyzed degradation of thiamin in the reaction mixture free of substrate-acceptor. Thiamin exhibited properties of both substrate-donor and -acceptor of the group transferred by thiaminase I. Identification of the products developed after thiaminase reaction involving thiamin was performed using chromatography and radiometry. Kinetics of the reaction is discussed.
