Ye B-Y, Shen W-L, Wang D, Li P, Zhang Z, Shi M-L, Zhang Y, Zhang F-X, Zhao Z-H
College of Life Sciences, Capital Normal University, 105 Xisanhuanbei Road, Haidian District, Beijing, 100048, China.
Beijing Institute of Biotechnology, No. 20, Dongdajie Street, Fengtai District, Beijing, 100071, P. R. China.
Mol Biol (Mosk). 2016 May-Jun;50(3):496-503. doi: 10.7868/S0026898416030034.
ZNF143 is a ubiquitously expressed transcription factor conserved in vertebrates and might regulate the expression of numerous genes. But its function in mediating chromatin interactions remains elusive. By integrated analysis of public datasets, we provided evidence that a majority of ZNF143 binding sites (BSs) were involved in CTCF-mediated chromatin interaction networks (CTCF-CINs) by overlapping with cohesin-BSs and CTCF-BSs. We further showed that only a very few CTCF-CINs were associated with ZNF143 alone, whereas those associated with ZNF143 and cohesin simultaneously were highly overlapped with constitutive, conserved CTCF-BSs and enriched at boundaries of chromatin topologically associating domains. These observations implicate that as an important partner of CTCF, ZNF143 helps it establish the conserved chromatin structure by cooperating with cohesin.
ZNF143是一种在脊椎动物中广泛表达且保守的转录因子，可能调控众多基因的表达。但其在介导染色质相互作用中的功能仍不清楚。通过对公共数据集的综合分析，我们提供了证据表明，大多数ZNF143结合位点（BSs）通过与黏连蛋白-BSs和CTCF-BSs重叠，参与了CTCF介导的染色质相互作用网络（CTCF-CINs）。我们进一步表明，只有极少数的CTCF-CINs单独与ZNF143相关，而那些同时与ZNF143和黏连蛋白相关的CTCF-CINs与组成型、保守的CTCF-BSs高度重叠，并富集在染色质拓扑相关结构域的边界。这些观察结果表明，作为CTCF的重要伙伴，ZNF143通过与黏连蛋白合作帮助其建立保守的染色质结构。
