Naoe T, Doi S, Yamanaka K, Naito K, Nitta M, Yamada K
Nihon Ketsueki Gakkai Zasshi. 1989 Feb;52(1):32-7.
Proto-oncogenes were studied by Southern blotting in 12 patients with chronic myelogenous leukemia in blast crisis (CML). Cytogenetically, all cases had Philadelphia (Ph1) chromosome and 4 had additional chromosomal abnormalities including +8, +19, i(17q), double Ph1. The bcr gene was rearranged in all cases and the region of breakage points were located in the 5.8 kb Bgl II-Bam HI fragment of bcr. In a case with double Ph1 the breakage points of bcr were thought to be different from each other. We analyzed other proto-oncogenes (N-ras, H-ras, c-myc and myb) by Southern blotting, but did not find any abnormalities.
采用Southern印迹法对12例慢性髓性白血病急变期（CML）患者的原癌基因进行了研究。细胞遗传学检查显示，所有病例均有费城（Ph1）染色体，4例有额外的染色体异常，包括+8、+19、i(17q)、双Ph1。所有病例中bcr基因均发生重排，断裂点位于bcr的5.8 kb Bgl II - Bam HI片段。在1例双Ph1病例中，bcr的断裂点被认为彼此不同。我们通过Southern印迹法分析了其他原癌基因（N-ras、H-ras、c-myc和myb），但未发现任何异常。
