Kitajima H, Takayama S, Yasunaga K
Nihon Ketsueki Gakkai Zasshi. 1989 Feb;52(1):93-101.
We studied the function of stored platelets by immunoelectron microscopy. The distribution of glycoprotein (GP) IIb/IIIa complex was demonstrated by the binding of a monoclonal anti-GP IIb/IIIa complex antibody (NNKY1-32) to platelets, and the binding of fibrinogen to platelets by use of an anti-fibrinogen antibody. The binding of NNKY1-32 decreased significantly in unstimulated platelets after storage for 5 days. When platelet aggregation was tested, 5-day-stored platelets did not aggregate in the presence of either ADP or epinephrine alone (40 microM each) or in combination (8 microM each), but did aggregate on stimulation with the combination of collagen and epinephrine (1 micrograms/ml and 1 microM, respectively). The 5-day-stored platelets stimulated with the combination of ADP and epinephrine scarcely bound fibrinogen, but did bind NNKY1-32. On stimulation with collagen and epinephrine, a large amount of fibrinogen bound to the surface membrane of 3- and 5-day-stored platelets, and the binding of NNKY1-32 also increased, reaching the same level seen in stored platelets stimulated with ADP and epinephrine. Our analysis of stored platelets revealed that the binding of NNKY1-32 decreased after storage for 5 days, but the binding increased even with subaggregating stimulation. The binding of fibrinogen correlated well with the rate of maximal platelet aggregation.
我们通过免疫电子显微镜研究了储存血小板的功能。通过单克隆抗糖蛋白（GP）IIb/IIIa复合物抗体（NNKY1-32）与血小板的结合来证明GP IIb/IIIa复合物的分布，并使用抗纤维蛋白原抗体来证明纤维蛋白原与血小板的结合。储存5天后，未刺激的血小板中NNKY1-32的结合显著减少。在检测血小板聚集时，储存5天的血小板在单独存在ADP或肾上腺素（各40 microM）或两者组合（各8 microM）时不聚集，但在胶原和肾上腺素组合（分别为1微克/毫升和1 microM）刺激下会聚集。用ADP和肾上腺素组合刺激的储存5天的血小板几乎不结合纤维蛋白原，但会结合NNKY1-32。用胶原和肾上腺素刺激时，大量纤维蛋白原结合到储存3天和5天的血小板表面膜上，NNKY1-32的结合也增加，达到在用ADP和肾上腺素刺激的储存血小板中所见的相同水平。我们对储存血小板的分析表明，储存5天后NNKY1-32的结合减少，但即使在亚聚集刺激下结合也会增加。纤维蛋白原的结合与最大血小板聚集率密切相关。
