Lama Ang Dawa, Kim Jorma, Martiskainen Olli, Klemola Tero, Salminen Juha-Pekka, Tyystjärvi Esa, Niemelä Pekka, Vuorisalo Timo
Section of Biodiversity and Environmental Sciences, Department of Biology, University of Turku, 20014, Turku, Finland.
Laboratory of Organic Chemistry and Chemical Biology, Department of Chemistry, University of Turku, 20014, Turku, Finland.
J Plant Res. 2016 Nov;129(6):1141-1150. doi: 10.1007/s10265-016-0850-z. Epub 2016 Jul 14.
We studied the possible roles of flavonoids in the antioxidant and antiherbivore chemistry in Jatropha curcas (L.), a Latin American shrub that holds great potential as a source of biofuel. Changes in flavonoid concentrations in the leaves of J. curcas seedlings exposed to artificial damage and to different rainfall patterns were assessed by applying a 3-factorial experiment in a greenhouse. The concentrations of different flavonoids in the leaves of seedlings were significantly affected by interaction effects of artificial damage, drought stress and age of the seedling. The highest flavonoid concentrations were obtained in seedlings imposed to the highest percentage of artificial damage (50 %) and grown under extreme drought stress (200 mm year). In this treatment combination, flavonoid concentrations were three-fold as compared to seedlings exposed to the same level of artificial damage but grown in 1900 mm year rainfall application. Without artificial damage, the concentration of flavonoids in the seedlings grown in 200 mm year rainfall application was still two-fold compared to seedlings grown in higher (>800 mm year) rainfall applications. Thus, the observed flavonoid concentration patterns in the leaves of J. curcas seedlings were primarily triggered by drought stress and light rather than by artificial damage, suggesting that drought causes oxidative stress in J. curcas.
我们研究了黄酮类化合物在麻疯树（一种具有巨大生物燃料来源潜力的拉丁美洲灌木）的抗氧化和抗食草动物化学中的可能作用。通过在温室中进行三因素实验，评估了遭受人工损伤和不同降雨模式的麻疯树幼苗叶片中黄酮类化合物浓度的变化。幼苗叶片中不同黄酮类化合物的浓度受到人工损伤、干旱胁迫和幼苗年龄的交互作用的显著影响。在遭受最高百分比人工损伤（50%）并在极端干旱胁迫（每年200毫米）下生长的幼苗中，黄酮类化合物浓度最高。在这种处理组合下，与遭受相同水平人工损伤但在每年1900毫米降雨量条件下生长的幼苗相比，黄酮类化合物浓度高出三倍。在没有人工损伤的情况下，每年降雨量为200毫米条件下生长的幼苗中黄酮类化合物浓度仍比在较高（每年>800毫米）降雨量条件下生长的幼苗高出两倍。因此，在麻疯树幼苗叶片中观察到的黄酮类化合物浓度模式主要是由干旱胁迫和光照引发的，而非人工损伤，这表明干旱会在麻疯树中引起氧化应激。
