通过测量甲氨蝶呤（DDMP）和依托泊苷（DDEP）对脱氧尿苷掺入人白血病细胞DNA的抑制作用来进行比较1,2,3 。
Comparison of metoprine (DDMP) and etoprine (DDEP) by measuring the inhibition of deoxyuridine incorporation into the DNA of human leukemic cells 1,2,3.
作者信息
Laszlo J, Fyfe M J, Swedwick D, Lee L, Brown O
出版信息
Cancer Treat Rep. 1978 Mar;62(3):341-4.
In vitro studies were used to measure the incorporation of labeled deoxyuridine into the DNA of human leukemic cells in autologous plasma in order to compare the inhibitory effects of two diaminopyrimidines, metoprine and etoprine. The results indicate that these two lipid-soluble folate antagonists give similar qualitative and quantitative effects over a concentration range of from 1 to 100 micrometer. However, drug-induced inhibition of deoxyuridine incorporation into DNA was quite variable for cells obtained from different adult patients, even within a given morphologic type of leukemia. Despite these biologic varitions in leukemic sensitivity in vitro, both drugs gave virtually identical results on an equimolar basis.
进行体外研究以测量标记的脱氧尿苷掺入自体血浆中人类白血病细胞的DNA，以便比较两种二氨基嘧啶（美托普林和依托普林）的抑制作用。结果表明，在1至100微米的浓度范围内，这两种脂溶性叶酸拮抗剂具有相似的定性和定量效果。然而，对于从不同成年患者获得的细胞，即使在给定形态学类型的白血病中，药物诱导的脱氧尿苷掺入DNA的抑制作用也有很大差异。尽管白血病细胞体外敏感性存在这些生物学差异，但两种药物在等摩尔基础上给出了几乎相同的结果。
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