Polat Nihat, Gunduz Abuzer, Gunduz Ayten, Cumurcu Tongabay, Gunduz Göksel
a Department of Ophthalmology , Medical Faculty, Inonu University , Malatya , Turkey.
b Department of Microbiology , Malatya State Hospital , Malatya , Turkey.
Curr Eye Res. 2017 Mar;42(3):364-367. doi: 10.1080/02713683.2016.1196710. Epub 2016 Jul 15.
To investigate the influence of corneal collagen crosslinking (CXL) on conjunctival flora.
Included in this study were 42 patients, all of whom were treated with CXL for progressive keratoconus (KC). Conjunctival swab samples were collected under operating-room conditions immediately before and immediately after the procedure. Samples were quickly immersed in Amies Transport Medium (ATM), transferred to the microbiology laboratory, diluted, and inoculated into three areas each of blood agar, chocolate agar, eosin methylene blue (EMB) agar, and Saboraud-Dextrose agar plates. Inoculated plates were incubated at 35°C for 24-48 h. Preliminary species identification of isolated bacterial colonies was based on catalase and oxidase tests, Gram staining, and colony morphology. Definitive identification of isolates was made with Vitek-II fully automated identification kits.
Cultures were positive in 24 (57.1%) preoperative and 10 (23.8%) postoperative samples (p ˂ 0.05). Microorganisms isolated from preoperative conjunctival samples included coagulase-negative Staphylococcus (CNS) spp. in 23 (54.8%) samples, S. aureus in three (7.1%), Moraxella spp. in two (4.8%), Streptococcus spp. in three (7.1%), Corynebacterium spp, in one (2.4%), Micrococcus spp. in one (2.4%), and Candida spp. in one (2.4%). Microorganisms isolated from postoperative conjunctival samples included CNS spp. in nine (21.4%) samples, S. aureus in one (2.4%), Corynebacterium spp. in two (4.8%), and Acinetobacter spp. in one (2.4%).
CXL appears to have a significant impact on conjunctiva flora.
研究角膜胶原交联（CXL）对结膜菌群的影响。
本研究纳入42例患者，均接受CXL治疗进行性圆锥角膜（KC）。在手术室条件下，于手术前及手术后立即采集结膜拭子样本。样本迅速浸入阿氏运送培养基（ATM）中，转移至微生物实验室，进行稀释，然后接种到血琼脂、巧克力琼脂、伊红美蓝（EMB）琼脂和沙氏葡萄糖琼脂平板的三个区域。接种后的平板在35°C下孵育24 - 48小时。基于过氧化氢酶和氧化酶试验、革兰氏染色及菌落形态对分离出的细菌菌落进行初步菌种鉴定。使用Vitek-II全自动鉴定试剂盒对分离株进行最终鉴定。
术前样本培养阳性的有24例（57.1%），术后样本培养阳性的有10例（23.8%）（p ˂ 0.05）。术前结膜样本中分离出的微生物包括凝固酶阴性葡萄球菌（CNS）属，共23例（54.8%）样本；金黄色葡萄球菌3例（7.1%）；莫拉菌属2例（4.8%）；链球菌属3例（7.1%）；棒状杆菌属1例（2.4%）；微球菌属1例（2.4%）；念珠菌属1例（2.4%）。术后结膜样本中分离出的微生物包括CNS属9例（21.4%）样本；金黄色葡萄球菌1例（2.4%）；棒状杆菌属2例（4.8%）；不动杆菌属1例（2.4%）。
CXL似乎对结膜菌群有显著影响。
