β,γ-修饰的α-L-苏型呋喃核糖基胸腺嘧啶三磷酸类似物的合成与聚合酶掺入
Synthesis and polymerase incorporation of β,γ-modified α-l-threofuranosyl thymine triphosphate mimics.
作者信息
Chen Zhe, Meek Kirsten N, Rangel Alexandra E, Heemstra Jennifer M
机构信息
Department of Chemistry and the Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, USA.
Department of Chemistry and the Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, USA.
出版信息
Bioorg Med Chem Lett. 2016 Aug 15;26(16):3958-62. doi: 10.1016/j.bmcl.2016.07.008. Epub 2016 Jul 4.
Three β,γ-modified α-l-threofuranosyl nucleoside triphosphates were synthesized. The β,γ-modified tTTPs undergo a single incorporation event with HIV RT but undergo multiple incorporations to form full-length product with engineered thermophilic polymerases.
合成了三种β,γ-修饰的α-L-苏糖呋喃糖基核苷三磷酸。β,γ-修饰的胸苷三磷酸（tTTPs）与HIV逆转录酶（RT）发生单次掺入事件，但与工程化嗜热聚合酶发生多次掺入以形成全长产物。
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