Dudley Emily S, Johnson Robert A, French DeAnne C, Boivin Gregory P
Department of Pathology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio, USA. Emily.
Laboratory Animal Resources, Wright State University, Dayton, Ohio, USA.
J Am Assoc Lab Anim Sci. 2016;55(4):443-50.
Tail biopsy is a common procedure that is performed to obtain genetic material for determining genotype of transgenic mice. The use of anesthetics or analgesics is recommended, although identifying safe and effective drugs for this purpose has been challenging. We evaluated the effects of topical 2.5% lidocaine-2.5% prilocaine cream applied to the distal tail tip at 5 or 60 min before biopsy, immersion of the tail tip for 10 seconds in ice-cold 70% ethanol just prior to biopsy, and immersion of the tail tip in 0.5% bupivacaine for 30 s after biopsy. Mice were 7, 11, or 15 d old at the time of tail biopsy. Acute behavioral responses, plasma corticosterone, and blood glucose were measured after biopsy, and body weight and performance in elevated plus maze and open-field tests after weaning. Ice-cold ethanol prior to biopsy prevented acute behavioral responses to biopsy, and both ice-cold ethanol and bupivacaine prevented elevations in corticosterone and blood glucose after biopsy. Tail biopsy with or without anesthesia did not affect body weight or performance on elevated plus maze or open-field tests. We recommend the use of ice-cold ethanol for topical anesthesia prior to tail biopsy in mice 7 to 15 d old.
尾部活检是一种常见的操作，用于获取遗传物质以确定转基因小鼠的基因型。虽然确定用于此目的的安全有效药物具有挑战性，但仍建议使用麻醉剂或镇痛药。我们评估了在活检前5分钟或60分钟将2.5%利多卡因-2.5%丙胺卡因霜局部应用于尾尖远端、在活检前将尾尖在冰冷的70%乙醇中浸泡10秒以及在活检后将尾尖在0.5%布比卡因中浸泡30秒的效果。在进行尾部活检时，小鼠的年龄为7、11或15天。在活检后测量急性行为反应、血浆皮质酮和血糖，并在断奶后测量体重以及在高架十字迷宫和旷场试验中的表现。活检前的冰冷乙醇可防止对活检的急性行为反应，冰冷乙醇和布比卡因均可防止活检后皮质酮和血糖升高。有无麻醉的尾部活检均不影响体重或在高架十字迷宫或旷场试验中的表现。我们建议在对7至15日龄的小鼠进行尾部活检前使用冰冷乙醇进行局部麻醉。
