Richter Zsófia, Janszky József, Sétáló György, Horváth Réka, Horváth Zsolt, Dóczi Tamás, Seress László, Ábrahám Hajnalka
Central Electron Microscopic Laboratory, University of Pécs Medical School, Pécs, Hungary.
Department of Neurology, University of Pécs Medical School, Pécs, Hungary; MTA-PTE Clinical Neuroscience MR Research Group, University of Pécs, Pécs, Hungary.
Neuroscience. 2016 Oct 1;333:140-50. doi: 10.1016/j.neuroscience.2016.07.011. Epub 2016 Jul 14.
The aim of the present work was to characterize neurons in the archi- and neocortical white matter, and to investigate their distribution in mesial temporal sclerosis. Immunohistochemistry and quantification of neurons were performed on surgically resected tissue sections of patients with therapy-resistant temporal lobe epilepsy. Temporal lobe tissues of patients with tumor but without epilepsy and that from autopsy were used as controls. Neurons were identified with immunohistochemistry using antibodies against NeuN, calcium-binding proteins, transcription factor Tbr1 and neurofilaments. We found significantly higher density of neurons in the archi- and neocortical white matter of patients with temporal lobe epilepsy than in that of controls. Based on their morphology and neurochemical content, both excitatory and inhibitory cells were present among these neurons. A subset of neurons in the white matter was Tbr-1-immunoreactive and these neurons coexpressed NeuN and neurofilament marker SMI311R. No colocalization of Tbr1 was observed with the inhibitory neuronal markers, calcium-binding proteins. We suggest that a large population of white matter neurons comprises remnants of the subplate. Furthermore, we propose that a subset of white matter neurons was arrested during migration, highlighting the role of cortical maldevelopment in epilepsy associated with mesial temporal sclerosis.
本研究的目的是对古皮质和新皮质白质中的神经元进行特征描述，并研究它们在颞叶内侧硬化中的分布。对药物难治性颞叶癫痫患者手术切除的组织切片进行免疫组织化学和神经元定量分析。患有肿瘤但无癫痫患者的颞叶组织以及尸检组织用作对照。使用抗NeuN、钙结合蛋白、转录因子Tbr1和神经丝的抗体通过免疫组织化学鉴定神经元。我们发现，颞叶癫痫患者的古皮质和新皮质白质中的神经元密度显著高于对照组。根据其形态和神经化学含量，这些神经元中既有兴奋性细胞也有抑制性细胞。白质中的一部分神经元对Tbr-1呈免疫反应性，这些神经元共表达NeuN和神经丝标志物SMI311R。未观察到Tbr1与抑制性神经元标志物钙结合蛋白的共定位。我们认为大量白质神经元是板下带的残余。此外，我们提出一部分白质神经元在迁移过程中停滞，突出了皮质发育异常在与颞叶内侧硬化相关的癫痫中的作用。
