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传统石榴汁与有机石榴汁的生物活性:抗氧化及抗诱变潜力

Biological Activity of Conventional and Organic Pomegranate Juices: Antioxidant and Antimutagenic Potential.

作者信息

Cano-Lamadrid M, Marhuenda-Egea F C, Hernández F, Rosas-Burgos E C, Burgos-Hernández A, Carbonell-Barrachina A A

机构信息

Departamento Tecnología Agroalimentaria, Grupo "Calidad y Seguridad Alimentaria (CSA), Escuela Politécnica Superior de Orihuela (EPSO), Universidad Miguel Hernández de Elche (UMH), Carretera de Beniel, km 3.2, 03312-Orihuela, Alicante, Spain.

Department of Agrochemistry and Biochemistry, University of Alicante, PO Box 99, 03080, Alicante, Spain.

出版信息

Plant Foods Hum Nutr. 2016 Dec;71(4):375-380. doi: 10.1007/s11130-016-0569-y.

Abstract

None of the health claims about pomegranate juices has been approved yet by European Food Safety Authority (EFSA). There is a general perception among consumers that organic foods are healthier, tastier, and more nutritive than the conventional products. The aim of this research was to study the differences in the biological activity between ready-for-consumption juices obtained from pomegranates fruits grown under conventional and organic agricultural practices. Antioxidant activity has been evaluated by three methods (DPPH, ABTS, and FRAP), together with the total contents of phenolics and punicalagin (HPLC-DAD); besides, the Ames test was used to evaluate the antimutagenic potential of the juices. Pomegranate juice, either from conventionally or organically grown fruits, was antimutagenic (mean of 51 and 90 % for Salmonella typhimurium TA100 and TA98, respectively) and it was capable of protecting DNA from both, base-pair or frame-shift type of mutations. In fact, the antimutagenicity of conventional pomegranate juice was higher than that achieved by the organic sample; this finding was linked to a higher punicalagin content (201 and 104 mg L for conventional and organic juices, respectively).

摘要

欧洲食品安全局(EFSA)尚未批准任何有关石榴汁的健康声明。消费者普遍认为有机食品比传统产品更健康、更美味且更具营养。本研究的目的是研究在传统和有机农业实践下种植的石榴所制得的即饮果汁之间的生物活性差异。通过三种方法(DPPH、ABTS和FRAP)评估了抗氧化活性,并测定了酚类物质和石榴皮苷的总含量(HPLC-DAD);此外,采用Ames试验评估了果汁的抗诱变潜力。无论是传统种植还是有机种植的石榴所制得的果汁均具有抗诱变作用(鼠伤寒沙门氏菌TA100和TA98的平均抗诱变率分别为51%和90%),并且能够保护DNA免受碱基对或移码类型的突变影响。事实上,传统石榴汁的抗诱变能力高于有机样品;这一发现与较高的石榴皮苷含量有关(传统果汁和有机果汁中石榴皮苷的含量分别为201 mg/L和104 mg/L)。

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