Soltani M, Sahingil D, Gokce Y, Hayaloglu A A
Department of Food Sciences & Technology, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University, 19395-6466 Tehran-Iran (IAUPS).
Department of Food Engineering, Faculty of Engineering, Inonu University, 44280 Malatya, Turkey.
J Dairy Sci. 2016 Oct;99(10):7744-7754. doi: 10.3168/jds.2016-11179. Epub 2016 Aug 8.
The effect of using various combinations of Rhizomucor miehei protease and camel chymosin (100:0, 75:25, 50:50, 25:75, and 0:100, respectively) on volatile composition and sensory scores in Iranian ultrafiltered white cheese was studied during 90d of ripening. A solid-phase microextraction-gas chromatography-mass spectrometric method was used for determining the volatile compounds of the cheeses. Forty compounds including esters (12), acids (6), ketones (9), alcohols (3), and miscellaneous compounds (10) were identified. The main classes of volatile components in the cheeses are esters, miscellaneous compounds, and ketones. The type and concentration of the coagulants influenced both volatile composition and sensory scores of the cheeses. Principal component analysis separated the cheeses based on the use of 2 coagulants in various combinations and ripening time. The cheeses produced using higher concentrations of R. miehei were separately located on the plot compared with the cheeses produced using higher concentrations of camel chymosin. Sensory evaluation of the cheeses showed that, in general, the cheeses produced using higher concentrations of camel chymosin received higher body and texture and odor and flavor scores than the cheese produced using higher concentrations of R. miehei. In conclusion, 2 combinations of R. miehei and camel chymosin (75:25 and 25:75, respectively) can be successfully used for the production of Iranian ultrafiltered white cheese, considering the results of volatile composition and sensory analysis.
研究了在90天的成熟过程中,使用米黑根毛霉蛋白酶和骆驼凝乳酶的不同组合(分别为100:0、75:25、50:50、25:75和0:100)对伊朗超滤白奶酪挥发性成分和感官评分的影响。采用固相微萃取-气相色谱-质谱法测定奶酪中的挥发性化合物。共鉴定出40种化合物,包括酯类(12种)、酸类(6种)、酮类(9种)、醇类(3种)和其他化合物(10种)。奶酪中挥发性成分的主要类别是酯类、其他化合物和酮类。凝乳剂的类型和浓度影响了奶酪的挥发性成分和感官评分。主成分分析根据两种凝乳剂的不同组合使用情况和成熟时间对奶酪进行了区分。与使用较高浓度骆驼凝乳酶生产的奶酪相比,使用较高浓度米黑根毛霉生产的奶酪在图中单独分布。奶酪的感官评价表明,总体而言,使用较高浓度骆驼凝乳酶生产的奶酪在质地、气味和风味方面的评分高于使用较高浓度米黑根毛霉生产的奶酪。总之,考虑到挥发性成分和感官分析的结果,米黑根毛霉和骆驼凝乳酶的两种组合(分别为75:25和25:75)可成功用于生产伊朗超滤白奶酪。