Razeghi Jahromi Soodeh, Ghaemi Amir, Alizadeh Akram, Sabetghadam Fatemeh, Moradi Tabriz Hedieh, Togha Mansoureh
MS Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran AND Department of Clinical Nutrition and Dietetics, Faculty of Nutrition and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Shefa Neuroscience Research Centre, Khatam-Alanbia Hospital, Tehran, Iran.
Department of Virology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran AND Shefa Neuroscience Research Centre, Khatam-Alanbia Hospital, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2016 Jun;15(3):212-9.
Several religions recommend periods of fasting. One of the most frequently asked questions of MS patients before the holy month of Ramadan is weather fasting might have an unfavorable effect on their disease course. This debate became more challenging after the publication of experimental studies suggesting that calorie restriction prior to disease induction attenuates disease severity. We conducted this study to assess early and late effects of fasting on the animal model of MS, known as autoimmune encephalomyelitis. EAE was induced in the C57BL/6 mice, using Myelin Oligodendrocyte Glycopeptide (MOG) 35-55 and they fasted every other day either after the appearance of the first clinical sign or 30 days after disease induction for ten days. Thereafter, the mice were sacrificed for further histological and immunological evaluations. Intermittent fasting after the establishment of EAE did not have any unfavorable effect on the course of disease. Moreover, fasting at the early phase of disease alleviated EAE severity by ameliorating spinal cord demyelination. Fasting suppressed the secretion of IFN-γ, TNF-α and raised IL-10 production in splenocytes. Fasting was also associated with a lower percent of cytotoxicity. Intermittent fasting not only had no unfavorable effect on EAE but also reduced EAE severity if started at early phase of disease.
几种宗教都提倡禁食期。在斋月这个圣月之前，多发性硬化症患者最常被问到的问题之一是禁食是否可能对他们的病程产生不利影响。在一些实验研究发表后，这场辩论变得更具挑战性，这些研究表明在疾病诱发前限制热量摄入可减轻疾病严重程度。我们进行这项研究以评估禁食对多发性硬化症动物模型（即自身免疫性脑脊髓炎）的早期和晚期影响。使用髓鞘少突胶质细胞糖蛋白（MOG）35 - 55在C57BL/6小鼠中诱发自身免疫性脑脊髓炎，然后在出现首个临床症状后或疾病诱发30天后每隔一天禁食十天。此后，处死小鼠以进行进一步的组织学和免疫学评估。自身免疫性脑脊髓炎形成后间歇性禁食对病程没有任何不利影响。此外，在疾病早期禁食通过改善脊髓脱髓鞘减轻了自身免疫性脑脊髓炎的严重程度。禁食抑制了脾细胞中γ干扰素、肿瘤坏死因子-α的分泌，并提高了白细胞介素-10的产生。禁食还与较低的细胞毒性百分比相关。间歇性禁食不仅对自身免疫性脑脊髓炎没有不利影响，而且如果在疾病早期开始还会降低自身免疫性脑脊髓炎的严重程度。
