Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Biosens Bioelectron. 2016 Dec 15;86:439-445. doi: 10.1016/j.bios.2016.06.089. Epub 2016 Jun 30.
In this work, a label-free photoelectrochemical (PEC) aptasensor was developed for adenosine detection based on CdS/PPy/g-C3N4 nanocomposites. The CdS/g-C3N4 heterojunction effectively prevented the photogenerated charges recombination of g-C3N4 and self-photocorrosion processes of CdS, improving photo-to-current conversion efficiency. The introduced polypyrrole (PPy) nanoparticles could lead to a more effective separation of photogenerated charges, thus resulting in a further increasing of photocurrent. The CdS/PPy/g-C3N4 was firstly employed as the photoactive materials for fabrication of aptasensor, and SH-aptamer was then adsorbed on the CdS/PPy/g-C3N4 modified electrodes through S-Cd bond. With increasing of adenosine concentration, the photocurrent decreased as the formation of SH-aptamer-adenosine bioaffinity complexes. Under optimal conditions, the PEC aptasensor had a sensitive response to adenosine in a linear range of 0.3nmolL(-1) to 200nmolL(-1) with a detection limit of 0.1nmolL(-1). Besides, the as-proposed aptasensor has also been applied in human serum samples analysis. The aptasensor exhibits high sensitivity and good stability, thus opening up a new promising PEC platform for some other small molecules analysis.
在这项工作中，基于 CdS/PPy/g-C3N4 纳米复合材料，开发了一种用于腺苷检测的无标记光电化学（PEC）适体传感器。CdS/g-C3N4 异质结有效阻止了 g-C3N4 的光生电荷复合和 CdS 的自光腐蚀过程，提高了光电转换效率。引入的聚吡咯（PPy）纳米粒子可以导致光生电荷更有效地分离，从而进一步增加光电流。CdS/PPy/g-C3N4 首先被用作制备适体传感器的光活性材料，然后 SH-适体通过 S-Cd 键吸附在 CdS/PPy/g-C3N4 修饰电极上。随着腺苷浓度的增加，由于 SH-适体-腺苷生物亲和复合物的形成，光电流减小。在最佳条件下，PEC 适体传感器对腺苷在 0.3nmolL(-1)至 200nmolL(-1)的线性范围内具有灵敏的响应，检测限为 0.1nmolL(-1)。此外，所提出的适体传感器还已应用于人血清样品分析。该适体传感器具有高灵敏度和良好的稳定性，为其他一些小分子的分析开辟了一个新的有前途的 PEC 平台。
