Castaño-Miquel Laura, Lois L Maria
Development Program, CRAG (CSIC-IRTA-UAB-UB), Edifici CRAG-Campus UAB, Bellaterra (Cerdanyola del Vallés), 08193, Barcelona, Spain.
Methods Mol Biol. 2016;1450:107-23. doi: 10.1007/978-1-4939-3759-2_9.
Plants display a high diversification degree of the SUMO conjugation machinery, which could confer a biological specialization of the different isoforms. For instance, the two essential Arabidopsis SUMO isoforms, SUMO1/2, display the highest conjugation rate when compared to SUMO3 and 5, suggesting that their specific biochemical properties may be linked to their biological specialization. In order to study the biochemical properties of plant SUMO conjugation systems, quantitative biochemical assays must be performed. We will present a detailed protocol for reconstituting an in vitro SUMO conjugation assay covering all steps from protein preparation to assay development.
植物的SUMO缀合机制呈现出高度的多样化程度，这可能赋予了不同异构体生物学特异性。例如，与SUMO3和5相比，拟南芥中两种必需的SUMO异构体SUMO1/2表现出最高的缀合率，这表明它们特定的生化特性可能与其生物学生物学特异性生物学特异性相关。为了研究植物SUMO缀合系统的生化特性，必须进行定量生化分析。我们将提供一份详细的方案，用于重建体外SUMO缀合分析，涵盖从蛋白质制备到分析开发的所有步骤。
