Wu Wenxiao, Chen Laizhong, Li Jing, Du Lupei, Li Minyong
Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of Pharmacy, Shandong University, Jinan, Shandong, 250012, China.
Methods Mol Biol. 2016;1461:91-9. doi: 10.1007/978-1-4939-3813-1_7.
Bioluminescence is a process that converts biochemical energy to visible light. Bioluminescence-based imaging technology has been widely applicable in the imaging of process envisioned in life sciences. As one of the most popular bioluminescence system, the firefly luciferin-luciferase system is exceptionally suitable for deep tissue imaging in living animals owing to its long wavelength emission light. Herein, we report the experimental detail of bioluminogenic imaging of aminopeptidase N activity both in vitro and in vivo.
生物发光是一种将生化能量转化为可见光的过程。基于生物发光的成像技术已广泛应用于生命科学所设想的过程的成像中。作为最流行的生物发光系统之一，萤火虫荧光素-荧光素酶系统由于其发射光波长较长，特别适合于活体动物的深部组织成像。在此，我们报告了体外和体内氨肽酶N活性生物发光成像的实验细节。
