Kumar Sunil, Lau Raymond, Hall Christopher E, Palaia Thomas, Rideout Drew A, Brathwaite Collin E, Ragolia Louis
Department of Biomedical Research, Winthrop University Hospital, Mineola, New York.
Department of Surgery, Winthrop University Hospital, Mineola, New York; Department of Endocrinology, Winthrop University Hospital, Mineola, New York.
Surg Obes Relat Dis. 2016 Sep-Oct;12(8):1523-1531. doi: 10.1016/j.soard.2016.04.007. Epub 2016 Apr 7.
Vertical sleeve gastrectomy (VSG) ameliorates metabolic complications in obese and diabetic patients through unknown mechanisms.
The objective of this study was to investigate the role of lipocalin-type prostaglandin D synthase (L-PGDS) in glucose regulation in response to VSG using L-PGDS knock-out (KO), knock-in (KI), and C57BL/6 (wild type) mice.
Winthrop University Hospital Research Institute.
Animals were divided into 6 groups: L-PGDS KO sham/VSG (n = 5), L-PGDS KI sham/VSG (n = 5), and C57BL/6 (wild type) sham/VSG (n = 5). Related parameters were measured in fasting animals after 10 weeks.
Our intraperitoneal glucose tolerance tests and homeostatic model assessment insulin resistance results showed significant glycemic improvement 10 weeks post-VSG in both C57BL/6 and KI groups compared with the sham group. In contrast, the KO group developed glucose intolerance and insulin resistance similar to or greater than the sham group 10 weeks post-VSG. Interestingly, weight gain was insignificant 10 weeks post-VSG in all the groups and even trended higher in the KO group compared with sham. Peptide YY levels in the KO group post-VSG were slightly increased but significantly less than other groups. Similarly, the KO group showed significantly less leptin sensitivity in response to VSG compared with the KI group. Total cholesterol level remained unchanged in all groups irrespective of sham or surgery but interestingly, the KO group had significantly higher cholesterol levels. In parallel, adipocyte size was also found to be significantly increased in the KO group post-VSG compared with the sham group.
Our findings propose that L-PGDS plays an important role in the beneficial metabolic effects observed after VSG.
垂直袖状胃切除术（VSG）可通过未知机制改善肥胖和糖尿病患者的代谢并发症。
本研究旨在利用脂蛋白D合成酶（L-PGDS）基因敲除（KO）、基因敲入（KI）和C57BL/6（野生型）小鼠，研究L-PGDS在VSG后血糖调节中的作用。
温思罗普大学医院研究所。
将动物分为6组：L-PGDS KO假手术/VSG组（n = 5）、L-PGDS KI假手术/VSG组（n = 5）和C57BL/6（野生型）假手术/VSG组（n = 5）。10周后对禁食动物测量相关参数。
我们的腹腔内葡萄糖耐量试验和稳态模型评估胰岛素抵抗结果显示，与假手术组相比，C57BL/6组和KI组在VSG后10周血糖有显著改善。相比之下，KO组在VSG后10周出现了与假手术组相似或更严重的葡萄糖不耐受和胰岛素抵抗。有趣的是，所有组在VSG后10周体重增加不显著，与假手术组相比，KO组甚至有更高的趋势。VSG后KO组的肽YY水平略有升高，但显著低于其他组。同样，与KI组相比，KO组对VSG的瘦素敏感性显著降低。无论假手术还是手术，所有组的总胆固醇水平均保持不变，但有趣的是，KO组的胆固醇水平显著更高。同时，与假手术组相比，VSG后KO组的脂肪细胞大小也显著增加。
我们的研究结果表明，L-PGDS在VSG后观察到的有益代谢效应中起重要作用。
