Divecha N, Mansouri H, Peat D, Cope G, Partridge L, McDonald C J
Department of Molecular Biology, University of Sheffield, Western Bank.
J Mol Endocrinol. 1989 Jul;3(1):7-14. doi: 10.1677/jme.0.0030007.
Members of the proline-rich protein (PRP) family of mouse parotid glands were analysed before and after stimulation with the beta-agonist isoprenaline by using a monoclonal antibody raised against the induced PRP A3(0) (GP-27). Antibody NAL1 reacted strongly with isoprenaline-induced B-type PRP precursors and their salivary counterparts, but not against the A-type PRPs A1(0) (Gp-66) and A2(0) (GP-45) or human salivary proteins, and it is likely that NAL1 recognizes a proline-rich repeat variant unique to this group of rodent PRPs. PRP-related antigens were observed in the parotid glands (N1(0) and N2(0] and saliva of normal mice. The antigens were located immunocytochemically in secretory granules of parotid acinar cells of both normal and isoprenaline-stimulated mice. The total amount of PRP antigens increased 16-fold from 2.5 to 40% of parotid protein after 10 days of isoprenaline treatment, as estimated by enzyme-linked immunosorbent assay. Immunoblotting showed that new PRP species appeared during the period of increase. After treatment with isoprenaline, B-type PRPs appeared first, followed by A3(0) and another member of the family. These results show that the mouse PRP family is larger than previously thought and can be divided immunologically into sub-groups. That a subset of PRPs are produced in the normal mouse indicates that there is differential beta-adrenergic regulation within the family, and also has implications for the role of PRPs in the normal maintenance of healthy dentition and other processes.
利用针对诱导型富脯氨酸蛋白A3(0)（GP - 27）产生的单克隆抗体，分析了β - 肾上腺素能激动剂异丙肾上腺素刺激前后小鼠腮腺中富脯氨酸蛋白（PRP）家族成员。抗体NAL1与异丙肾上腺素诱导的B型PRP前体及其唾液对应物强烈反应，但不与A型PRP的A1(0)（Gp - 66）和A2(0)（GP - 45）或人唾液蛋白反应，并且NAL1可能识别这组啮齿动物PRP特有的富脯氨酸重复变体。在正常小鼠的腮腺（N1(0)和N2(0)）和唾液中观察到PRP相关抗原。通过免疫细胞化学方法确定，这些抗原位于正常和异丙肾上腺素刺激小鼠的腮腺腺泡细胞的分泌颗粒中。用酶联免疫吸附测定法估计，异丙肾上腺素处理10天后，PRP抗原总量从腮腺蛋白的2.5%增加到40%，增加了16倍。免疫印迹显示，在增加期间出现了新的PRP种类。用异丙肾上腺素处理后，B型PRP首先出现，随后是A3(0)和该家族的另一个成员。这些结果表明，小鼠PRP家族比以前认为的更大，并且可以在免疫学上分为亚组。正常小鼠中产生一部分PRP，这表明该家族内存在β - 肾上腺素能差异调节，并且对PRP在健康牙列和其他过程的正常维持中的作用也有影响。
