不同脂蛋白类别中游离胆固醇的肝脏摄取与代谢。大鼠体内研究。
Hepatic uptake and metabolism of free cholesterol from different lipoprotein classes. An in vivo study in the rat.
作者信息
Bravo E, Cantafora A, Argiolas L
机构信息
Istituto Superiore di Sanita', Laboratory of Metabolism and Pathological Biochemistry, Rome, Italy.
出版信息
Biochim Biophys Acta. 1989 Jun 28;1003(3):315-20. doi: 10.1016/0005-2760(89)90238-5.
Hepatic metabolism of [14C]cholesterol, vehiculated by LDL, HDL2 and HDL3 lipoprotein particles, has been studied in rats with a permanent biliary drainage. The lipoprotein fractions were infused individually by a jugular vein catheter and bile was collected for 180 min after the administration. At the end of this period, the animals were killed and the blood and livers were collected. The free cholesterol of the HDL2 fraction was secreted into bile, mainly as bile salt, preferentially to that associated with HDL3 and LDL fractions (11.7% vs. 2.3% and 0.3%, respectively). The free cholesterol of the HDL3 fraction, on the other hand, was taken up by liver more quickly and in a higher proportion than that associated with other lipoprotein fractions. The label incorporation in this lipoprotein fraction was secreted earlier and not transformed into bile. The contribution of LDL-vehiculated free cholesterol to bile secretion was small and the hepatic uptake amounted to no more than 12% of the injected label.
在永久性胆汁引流的大鼠中，研究了由低密度脂蛋白（LDL）、高密度脂蛋白2（HDL2）和高密度脂蛋白3（HDL3）脂蛋白颗粒运载的[14C]胆固醇的肝脏代谢情况。通过颈静脉导管分别输注脂蛋白组分，并在给药后收集180分钟的胆汁。在此时间段结束时，处死动物并收集血液和肝脏。HDL2组分的游离胆固醇主要以胆汁盐的形式分泌到胆汁中，相比于与HDL3和LDL组分相关的游离胆固醇，其分泌比例更高（分别为11.7%、2.3%和0.3%）。另一方面，HDL3组分的游离胆固醇比与其他脂蛋白组分相关的游离胆固醇更快且以更高比例被肝脏摄取。该脂蛋白组分中的标记物掺入物分泌较早且未转化为胆汁。LDL运载的游离胆固醇对胆汁分泌的贡献较小，肝脏摄取量不超过注射标记物的12%。
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