Jové Mariona, Gatius Sònia, Yeramian Andree, Portero-Otin Manuel, Eritja Núria, Santacana Maria, Colas Eva, Ruiz Maria, Pamplona Reinald, Matias-Guiu Xavier
Department of Experimental Medicine, Universitat de Lleida, IRBLleida, E-25198, Lleida, Spain.
Department of Pathology and Molecular Genetics/Oncologic Pathology Group, Hospital Universitari Arnau de Vilanova, Universitat de Lleida, IRBLleida, E-25198, Lleida, Spain.
Oncotarget. 2016 Aug 9;7(32):52364-52374. doi: 10.18632/oncotarget.10564.
Metabolomics, an essential technique in precision medicine, contributes to the molecular fingerprinting of tumours, further helping to understand their pathogenesis. In this work, using a LC-ESI-QTOF-MS/MS platform, we demonstrated the existence of a specific metabolomic signature which could define endometrioid endometrial carcinoma (EEC), arising the endocannabinoid system as a potential pathway involved in EC pathogenesis. Metabolomics could also shed light in the processes involved in myometrial invasion, proposing new targets for possible therapeutic intervention. Consequently, we also described a different metabolomic profile in surface endometrioid carcinoma and myometrial invasive front. We validated pathways disclosed by metabolomics by immunohistochemistry. Specifically, endocannabinoid and purine metabolism could be involved in tumor myometrial invasion.
代谢组学是精准医学中的一项重要技术，有助于对肿瘤进行分子指纹识别，进一步帮助了解其发病机制。在这项工作中，我们使用液相色谱-电喷雾-四极杆飞行时间串联质谱（LC-ESI-QTOF-MS/MS）平台，证明了存在一种特定的代谢组学特征，该特征可以定义子宫内膜样子宫内膜癌（EEC），提示内源性大麻素系统是子宫内膜癌（EC）发病机制中涉及的潜在途径。代谢组学还可以阐明子宫肌层浸润所涉及的过程，提出可能的治疗干预新靶点。因此，我们还描述了表面子宫内膜样癌和子宫肌层浸润前沿的不同代谢组学特征。我们通过免疫组织化学验证了代谢组学揭示的通路。具体而言，内源性大麻素和嘌呤代谢可能参与肿瘤的子宫肌层浸润。
