从人毛囊中分离和培养神经嵴干细胞

Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles.

作者信息

Yang Ruifeng, Xu Xiaowei

机构信息

Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104, USA.

出版信息

Methods Mol Biol. 2016;1453:49-55. doi: 10.1007/978-1-4939-3786-8_7.

DOI:10.1007/978-1-4939-3786-8_7

PMID:27431246

Abstract

The hair follicle undergoes lifelong cycling and growth. Previous studies have been focused on epithelial stem cells in the hair follicles. Neural crest stem cells (NCSCs) are pluripotent cells that can persist in adult tissues. We have previously demonstrated that human NCSCs can be isolated from hair follicles. Here, we present a protocol to isolate NCSCs from human hair follicles based on their specific surface-marker expression of CD271/HNK1 or CD271/CD49D (alpha4 integrin). NCSCs can be expanded in the culture as neural spheres or attached cells.

摘要

毛囊经历终身循环和生长。以往的研究主要集中在毛囊中的上皮干细胞。神经嵴干细胞（NCSCs）是多能细胞，可存在于成体组织中。我们之前已经证明，人类神经嵴干细胞可以从毛囊中分离出来。在此，我们提出一种基于CD271/HNK1或CD271/CD49D（α4整合素）的特异性表面标志物表达从人毛囊中分离神经嵴干细胞的方案。神经嵴干细胞可以在培养中作为神经球或贴壁细胞进行扩增。