含表达巢蛋白绿色荧光蛋白的毛囊相关多能（HAP）干细胞的产毛小鼠触须毛囊的明胶海绵（®）长期组织培养方案。

Protocols for Gelfoam(®) Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles Containing Nestin-GFP-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells for Long Time Periods.

作者信息

Cao Wenluo, Liu Fang, Amoh Yasuyuki, Hoffman Robert M

机构信息

AntiCancer Inc., San Diego, CA, USA.

Department of Surgery, University of California, San Diego, San Diego, CA, USA.

出版信息

Methods Mol Biol. 2016;1453:145-50. doi: 10.1007/978-1-4939-3786-8_15.

DOI:10.1007/978-1-4939-3786-8_15

PMID:27431254

Abstract

Gelfoam(®)-histocultured whisker follicles from nestin-driven-green fluorescent protein (ND-GFP) mice produced growing pigmented and unpigmented hair shafts. Hair-shaft length increased rapidly by day 4 and continued growing until at least day 12 after which the hair-shaft length was constant. By day 63 in histoculture, the number of ND-GFP hair follicle-associated pluripotent (HAP) stem cells increased significantly and the follicles were intact. Three-dimensional Gelfoam(®) histoculture of hair follicles can provide a very long-term period for evaluating novel agents to promote hair growth.

摘要

来自巢蛋白驱动的绿色荧光蛋白（ND-GFP）小鼠的明胶海绵（®）组织培养的须毛囊产生了生长中的有色素和无色素毛干。到第4天时毛干长度迅速增加，并持续生长直至至少第12天，之后毛干长度保持恒定。在组织培养至第63天时，与ND-GFP毛囊相关的多能（HAP）干细胞数量显著增加，且毛囊完整。毛囊的三维明胶海绵（®）组织培养可为评估促进头发生长的新型药物提供非常长的时间周期。