改性磷酸钙处理对人牙根骨质的矿化及抗菌作用
Mineralising and antibacterial effects of modified calcium phosphate treatment on human root cementum.
作者信息
Gu Haijing, Ling Junqi, Zhou Xiaoyan, Liu Limin, Zhao Ziming, Gao Jin-Long
机构信息
Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University & Guangdong Provincial Key Laboratory of Stomatology, 56 Ling Yuan Xi Road, Guangzhou, 510055, China.
Department of Biomaterials & Biomimetics, New York University College of Dentistry, New York City, USA.
出版信息
BMC Oral Health. 2016 Jul 19;17(1):22. doi: 10.1186/s12903-016-0246-4.
BACKGROUND
Aging population will lead to the increase of incidence of root caries globally. The clinical management of root caries is challenging due to the difficulty in moisture isolation. The root caries is caused by the release of organic acids from cariogenic bacteria which results in the dissolution of cementum and dentin of the root. The purpose of this study is to study the efficacy of modified saturated calcium phosphate solution (CaP) supplement with zinc (Zn(2+)) and/or fluoride (F(-)) in providing root cementum surfaces less susceptible to acid dissolution and bacterial colonization.
METHODS
Human root cementum sections from extracted premolars were treated with three modified calcium phosphate solutions (M/A-CaPs) respectively: (A) CaP-F/Zn, supplemented with F(-) and Zn(2+); (B) CaP-F, supplemented with F(-) only; (C) CaP-Zn, supplemented with Zn(2+) only. The surface characteristics of treated cementum sections were investigated using scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FT-IR). Following the acid attack and Streptococcus mutans challenge, M/A-CaPs treated cementum surfaces were analysed using inductive coupled plasma (ICP) and SEM respectively.
RESULTS
Compared with the control group, M/A-CaPs treated cementum presented significant improvements in resistance to acid dissolution and bacterial colonization. Among M/A-CaPs, the CaP-F/Zn treated cementum surfaces released the lowest amount of Ca(2+) ions (2.11 ± 0.51 ppm) upon acid challenge (n = 3, p < 0.01) and also presented the most significant inhibiting effect against the colonization of S. mutans (n = 180, p < 0.05).
CONCLUSIONS
Saturated calcium phosphate solution CaP supplemented with both F(-) and Zn(2+) could be applied as an effective coating material providing acid resistance and antibacterial property on cementum surfaces. The modified calcium phosphate-based solution could be a new treatment strategy to prevent the development of root caries and arrest the further progression of root caries.
背景
全球人口老龄化将导致根龋发病率上升。由于难以隔离湿气，根龋的临床管理具有挑战性。根龋是由致龋菌释放有机酸引起的，这会导致牙根牙骨质和牙本质溶解。本研究的目的是研究添加锌（Zn(2+)）和/或氟（F(-)）的改良饱和磷酸钙溶液（CaP）在使牙根牙骨质表面不易被酸溶解和细菌定植方面的效果。
方法
分别用三种改良磷酸钙溶液（M/A-CaPs）处理从拔除的前磨牙获取的人牙根牙骨质切片：（A）CaP-F/Zn，添加F(-)和Zn(2+)；（B）CaP-F，仅添加F(-)；（C）CaP-Zn，仅添加Zn(2+)。使用扫描电子显微镜（SEM）和傅里叶变换红外光谱（FT-IR）研究处理后的牙骨质切片的表面特征。在进行酸侵蚀和变形链球菌攻击后，分别使用电感耦合等离子体（ICP）和SEM分析经M/A-CaPs处理的牙骨质表面。
结果
与对照组相比，经M/A-CaPs处理的牙骨质在抗酸溶解和细菌定植方面有显著改善。在M/A-CaPs中，经CaP-F/Zn处理的牙骨质表面在酸攻击后释放的Ca(2+)离子量最低（2.11±0.51 ppm）（n = 3，p < 0.01），并且对变形链球菌的定植也表现出最显著的抑制作用（n = 180，p < 0.05）。
结论
添加F(-)和Zn(2+)的饱和磷酸钙溶液CaP可作为一种有效的涂层材料，在牙骨质表面提供抗酸和抗菌性能。基于磷酸钙的改良溶液可能是预防根龋发展和阻止根龋进一步进展的一种新的治疗策略。
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