Niu Yuewei, Song Wantong, Zhang Dawei, Tang Zhaohui, Deng Mingxiao, Chen Xuesi
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; College of Chemistry, Northeast Normal University, Changchun 130024, PR China.
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
Acta Biomater. 2016 Oct 1;43:262-268. doi: 10.1016/j.actbio.2016.07.026. Epub 2016 Jul 16.
Photoacoustic imaging (PAI) is an emerging modality in biomedical imaging. Photoacoustic effect is the basis for PAI, where a photoacoustic contrast agent absorbs optical pulses to initiate localized heating and rapid thermal expansion, thus generating thermoelastic stress waves. Therefore, ideal PAI dyes should have strong NIR light absorbance and high light-heat conversion efficiency. However, most current low molecular weight organic PAI contrast agents are fluorescent dyes, where the light-heat conversion efficiency is dramatically impaired due to the energy loss by fluorescence emission. Herein, we report a series of highly efficient photoacoustic dyes with COOH, NH2 and NHS ester functionalities, from an inexpensive industrial computer-to-plate NIR absorber (IR830 p-toluenesulfonate) that has a strong NIR absorbance but an extremely low fluorescence emission. In vitro and in vivo studies show that the functional IR830 dyes have low cytotoxicity, and are 2.1 folds brighter in photoacoustic imaging than traditional photoacoustic dye indocyanine green (ICG). The Lowest Limit of Quantification of the IR830 series dyes is as low as the 1/7 of that of ICG. These indicate that the functional IR830 dyes have great potential as highly efficient photoacoustic dyes.
Photoacoustic imaging (PAI) is an emerging modality in biomedical imaging. Ideal PAI dyes should have strong NIR absorbance and high light-heat conversion efficiency. However, most current low molecular weight organic PAI contrast agents are fluorescent dyes, where the light-heat conversion efficiency is dramatically impaired due to the energy loss by fluorescence emission. Herein we report a series of highly efficient functional photoacoustic dyes from an inexpensive industrial computer-to-plate NIR absorber (IR830) that has a strong NIR absorbance but an extremely low fluorescence emission. The functional IR830 dyes show low cytotoxicity, much brighter in photoacoustic imaging than traditional photoacoustic dye indocyanine green. These indicate that the functional IR830 dyes have great potential as highly efficient photoacoustic dyes.
光声成像（PAI）是生物医学成像中的一种新兴模式。光声效应是PAI的基础，光声造影剂吸收光脉冲以引发局部加热和快速热膨胀，从而产生热弹性应力波。因此，理想的PAI染料应具有强近红外光吸收能力和高光热转换效率。然而，目前大多数低分子量有机PAI造影剂都是荧光染料，由于荧光发射导致能量损失，其光热转换效率显著受损。在此，我们报道了一系列具有COOH、NH2和NHS酯官能团的高效光声染料，它们源自一种廉价的工业计算机直接制版近红外吸收剂（IR830对甲苯磺酸盐），该吸收剂具有强近红外吸收能力但荧光发射极低。体外和体内研究表明，功能性IR830染料具有低细胞毒性，在光声成像中比传统光声染料吲哚菁绿（ICG）亮2.1倍。IR830系列染料的最低定量限低至ICG的1/7。这些表明功能性IR830染料作为高效光声染料具有巨大潜力。
光声成像（PAI）是生物医学成像中的一种新兴模式。理想的PAI染料应具有强近红外吸收能力和高光热转换效率。然而，目前大多数低分子量有机PAI造影剂都是荧光染料，由于荧光发射导致能量损失，其光热转换效率显著受损。在此我们报道了一系列源自廉价工业计算机直接制版近红外吸收剂（IR830）的高效功能性光声染料，该吸收剂具有强近红外吸收能力但荧光发射极低。功能性IR830染料显示出低细胞毒性，在光声成像中比传统光声染料吲哚菁绿亮得多。这些表明功能性IR830染料作为高效光声染料具有巨大潜力。
