Research Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Biomater Sci. 2018 May 29;6(6):1503-1516. doi: 10.1039/c8bm00104a.
Phototheranostic technology based on photoacoustic imaging (PAI) and photothermal therapy (PTT) is emerging as a powerful tool for tumor theranostic applications. For effective tumor eradication, a novel PAI/PTT theranostic nanoagent with an excellent optical absorption and photothermal capability is highly desired. Herein, we present a new PAI/PTT nanohybrid named sMoSe2-ICG NSs by covalently conjugating aminated indocyanine green (ICG) onto a single layer of molybdenum selenide nanosheets (sMoSe2 NSs). We first validate the sMoSe2-ICG NS agent for the PAI and PTT effect in vitro and then use it for highly-sensitive PAI guided highly efficient tumor PTT in vivo. The sMoSe2-ICG NS hybrid possesses several advantages for PAI/PTT applications: (1) the sMoSe2-ICG NSs have strong absorbance in the broad near-infrared (NIR) region, enabling a highly efficient PAI/PTT theranostic effect and the selection of the most widely used excitation wavelength of 808 nm for PTT; (2) the photothermal ability of ICG in sMoSe2-ICG NSs is augmented due to ICG aggregation induced fluorescence quenching and the re-absorbance of ICG fluorescence by sMoSe2 NSs, which further enhances the PAI/PTT theranostic effect. (3) The characteristic absorption peak of sMoSe2-ICG NSs is red-shifted compared to free ICG, resulting in a higher PAI signal-to-noise ratio (SNR) in vivo. Thus, combined with the good stability, high biocompatibility and minimal toxicity properties, the obtained sMoSe2-ICG NSs hybrid has bright prospects for use in future PAI/PTT clinical applications.
基于光声成像（PAI）和光热治疗（PTT）的光热治疗技术正在成为肿瘤治疗应用的有力工具。为了有效消除肿瘤，人们非常希望有一种具有优异光吸收和光热性能的新型 PAI/PTT 治疗纳米制剂。在此，我们通过将氨基化吲哚菁绿（ICG）共价接枝到单层二硒化钼纳米片（sMoSe2 NSs）上，制备了一种新型的 PAI/PTT 纳米杂化材料 sMoSe2-ICG NSs。我们首先在体外验证了 sMoSe2-ICG NS 试剂的 PAI 和 PTT 效应，然后将其用于体内高灵敏度 PAI 引导的高效肿瘤 PTT。sMoSe2-ICG NS 杂化物具有用于 PAI/PTT 应用的几个优点：（1）sMoSe2-ICG NSs 在宽近红外（NIR）区域具有很强的吸光度，能够实现高效的 PAI/PTT 治疗效果，并选择最广泛使用的 808nm 激发波长用于 PTT；（2）由于 ICG 聚集诱导荧光猝灭和 sMoSe2 NSs 对 ICG 荧光的再吸收，sMoSe2-ICG NSs 中 ICG 的光热能力增强，进一步增强了 PAI/PTT 治疗效果。（3）与游离 ICG 相比，sMoSe2-ICG NSs 的特征吸收峰红移，导致体内 PAI 信号-噪声比（SNR）更高。因此，结合良好的稳定性、高生物相容性和最小毒性，所得到的 sMoSe2-ICG NSs 杂化物在未来的 PAI/PTT 临床应用中具有广阔的前景。
