Chidley Hemangi G, Deshpande Ashish B, Oak Pranjali S, Pujari Keshav H, Giri Ashok P, Gupta Vidya S
Plant Molecular Biology Unit, Division of Biochemical Sciences, CSIR - National Chemical Laboratory, Pune 411 008, India.
Dr Balasaheb Sawant Konkan Agriculture University, Dapoli 415712, India.
J Sci Food Agric. 2017 Mar;97(5):1624-1633. doi: 10.1002/jsfa.7912. Epub 2016 Aug 9.
Ripening-associated softening is one of the important attributes that largely determines the shelf-life of mango (Mangifera indica Linn.) fruits. To reveal the effect of pre-climacteric ethylene treatment on ripening-related softening of Alphonso mango, ethylene treatment was given to mature, raw Alphonso fruits. Changes in the pool of reducing and non-reducing sugars, enzymatic activity of three glycosidases: β-d-galactosidase, α-d-mannosidase and β-d-glucosidase and their relative transcript abundance were analysed for control and ethylene treated fruits during ripening.
Early activity of all the three glycosidases and accelerated accumulation of reducing and non-reducing sugars on ethylene treatment was evident. β-d-Galactosidase showed the highest activity among three glycosidases in control fruits and marked increase in activity upon ethylene treatment. This was confirmed by the histochemical assay of its activity in control and ethylene treated ripe fruits. Relative transcript abundance revealed high transcript levels of β-d-galactosidase in control fruits. Ethylene-treated fruits showed early and remarkable increase in the β-d-galactosidase transcripts while α-d-mannosidase transcript variants displayed early accumulation.
The findings suggest reduction in the shelf-life of Alphonso mango upon pre-climacteric ethylene treatment, a significant role of β-d-galactosidase and α-d-mannosidase in the ripening related softening of Alphonso fruits and transcriptional regulation of their expression by ethylene. © 2016 Society of Chemical Industry.
与成熟相关的软化是很大程度上决定芒果（芒果属印度种林奈）果实货架期的重要特性之一。为揭示采前乙烯处理对阿方索芒果成熟相关软化的影响，对成熟的未成熟阿方索果实进行了乙烯处理。分析了对照果实和乙烯处理果实在成熟过程中还原糖和非还原糖库的变化、三种糖苷酶（β-d-半乳糖苷酶、α-d-甘露糖苷酶和β-d-葡萄糖苷酶）的酶活性及其相对转录丰度。
乙烯处理后，所有三种糖苷酶的早期活性以及还原糖和非还原糖的加速积累都很明显。β-d-半乳糖苷酶在对照果实的三种糖苷酶中活性最高，乙烯处理后活性显著增加。通过对对照果实和乙烯处理成熟果实中其活性的组织化学分析证实了这一点。相对转录丰度显示对照果实中β-d-半乳糖苷酶的转录水平较高。乙烯处理的果实中β-d-半乳糖苷酶转录本早期显著增加，而α-d-甘露糖苷酶转录变体则早期积累。
研究结果表明，采前乙烯处理会缩短阿方索芒果的货架期，β-d-半乳糖苷酶和α-d-甘露糖苷酶在阿方索果实成熟相关软化中起重要作用，且乙烯对其表达具有转录调控作用。© 2016 化学工业协会。
