Ishizaki K, Chang H R, Eguchi T, Ikenaga M
Radiation Biology Center, Kyoto University, Japan.
Cell Struct Funct. 1989 Apr;14(2):173-81. doi: 10.1247/csf.14.173.
High voltage electric pulses, 1000 V/cm, were found to induce cell fusion efficiently when delivered to cells growing in a monolayer culture. The maximum yield of fused cells was about 30% of cells remaining after treatment. Colonies of interspecies hybrid cells between mouse and others also appeared with a frequency of 3 X 10(-4) - 2 X 10(-5) after culturing fused cells in a selection medium that permits the growth of only hybrid cells. This method of electrofusion was applied for complementation analysis of DNA repair-deficient xeroderma pigmentosum cells. Efficient cell fusion was also observed with these human diploid fibroblasts and the resulting heterodikaryons showed a recovery of ultraviolet light-induced unscheduled DNA synthesis to the same extent as in normal cells.
发现当向单层培养生长的细胞施加1000 V/cm的高压电脉冲时，能有效地诱导细胞融合。融合细胞的最大产量约为处理后剩余细胞的30%。在仅允许杂交细胞生长的选择培养基中培养融合细胞后，小鼠与其他物种之间的种间杂交细胞集落也以3×10(-4)-2×10(-5)的频率出现。这种电融合方法被应用于DNA修复缺陷型着色性干皮病细胞的互补分析。在这些人类二倍体成纤维细胞中也观察到了高效的细胞融合，并且所产生的异核体显示紫外线诱导的非预定DNA合成恢复到与正常细胞相同的程度。
