在巴西亚马逊疟疾流行地区，细胞因子基因多态性与抗恶性疟原虫二磷酸果糖醛缩酶（anti-PvDBP）、抗恶性疟原虫裂殖子表面蛋白1（anti-PvAMA-1）或抗恶性疟原虫裂殖子表面蛋白119（anti-PvMSP-119）IgG抗体水平无关。
Cytokine gene polymorphisms are not associated with anti-PvDBP, anti-PvAMA-1 or anti-PvMSP-119 IgG antibody levels in a malaria-endemic area of the Brazilian Amazon.
作者信息
Furini Adriana A C, Capobianco Marcela P, Storti-Melo Luciane M, Cunha Maristela G, Cassiano Gustavo C, Machado Ricardo Luiz D
机构信息
Department of Dermatology, Infectious and Parasitic Diseases, São José do Rio Preto Medical School, São José do Rio Preto, São Paulo, Brazil.
Department of Biology, São Paulo State University, São José do Rio Preto, São Paulo, Brazil.
出版信息
Malar J. 2016 Jul 19;15(1):374. doi: 10.1186/s12936-016-1414-3.
BACKGROUND
The immune response against Plasmodium vivax immunogenic epitopes is regulated by pro- and anti-inflammatory cytokines that determine antibody levels and class switching. Cytokine gene polymorphisms may be responsible for changes in the humoral immune response against malaria. The aim of this study was to evaluate whether polymorphisms in the TNFA, IFNG and IL10 genes would alter the levels of anti-PvAMA1, PvDBP and -PvMSP-119 IgG antibodies in patients with vivax malaria.
METHODS
Samples from 90 vivax malaria-infected and 51 uninfected subjects from an endemic area of the Brazilian Amazon were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to identify polymorphisms of the genes TNFA (-1031T > C, -308G > A, -238G > A), IFNG (+874T > A) and IL10 (-819C > T, -592C > A). The levels of total IgG against PvAMA1, PvDBP and PvMSP-119 were determined using an enzyme-linked immunosorbent assay (ELISA). Associations between the polymorphisms and the antibody response were assessed by means of logistic regression models.
RESULTS
No significant differences were found in the levels of IgG antibodies against the PvAMA-1, PvDBP or PvMSP-119 proteins in relation to the studied polymorphisms.
CONCLUSIONS
Although no associations were found among the evaluated genotypes and alleles and anti-merozoite IgG class P. vivax antibody levels, this study helps elucidate the immunogenic profile involved in the humoral immune response in malaria.
背景
针对间日疟原虫免疫原性表位的免疫反应受促炎和抗炎细胞因子调节，这些细胞因子决定抗体水平和类别转换。细胞因子基因多态性可能导致针对疟疾的体液免疫反应发生变化。本研究旨在评估肿瘤坏死因子α（TNFA）、干扰素γ（IFNG）和白细胞介素10（IL10）基因的多态性是否会改变间日疟患者抗间日疟原虫顶端膜抗原1（PvAMA1）、间日疟原虫二硫键结合蛋白（PvDBP）和间日疟原虫裂殖子表面蛋白119（PvMSP-119）IgG抗体水平。
方法
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对来自巴西亚马逊地区一个流行区的90例间日疟感染患者和51例未感染受试者的样本进行基因分型，以鉴定TNFA基因（-1031T>C、-308G>A、-238G>A）、IFNG基因（+874T>A）和IL10基因（-819C>T、-592C>A）的多态性。使用酶联免疫吸附测定（ELISA）法测定抗PvAMA1、PvDBP和PvMSP-119的总IgG水平。通过逻辑回归模型评估多态性与抗体反应之间的关联。
结果
在所研究的多态性方面，抗PvAMA-1、PvDBP或PvMSP-119蛋白的IgG抗体水平未发现显著差异。
结论
尽管在所评估的基因型和等位基因与间日疟裂殖子IgG类抗体水平之间未发现关联，但本研究有助于阐明疟疾体液免疫反应中涉及的免疫原性特征。
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