在伊朗首都，Haarlem 3 是耐多药和广泛耐药结核分枝杆菌的主要基因型家族：一项为期 5 年的调查。
Haarlem 3 is the predominant genotype family in multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis in the capital of Iran: A 5-year survey.
机构信息
Mycobacteriology and Pulmonary Research Department, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave., Jomhhoori St., Tehran 1316943551, Iran; Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.
Mycobacteriology and Pulmonary Research Department, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave., Jomhhoori St., Tehran 1316943551, Iran.
出版信息
J Glob Antimicrob Resist. 2016 Jun;5:7-10. doi: 10.1016/j.jgar.2016.01.007. Epub 2016 Feb 26.
The objective of this study was to further understand the genetic diversity of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis isolates prevalent in Tehran, the capital city of Iran. From January 2010 to March 2015, a total of 723 M. tuberculosis strains were isolated from patients with pulmonary tuberculosis (TB). A total of 23 MDR, pre-XDR and XDR M. tuberculosis isolates were genotyped by spoligotyping and 24-loci mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) typing. The results showed that the MDR, pre-XDR and XDR M. tuberculosis strains mainly belonged to the Haarlem 3 genotype (11/23; 47.8%), followed by the Beijing family (9/23; 39.1%). In addition, the 23 strains were clustered into 21 genotypes using a 24-loci MIRU-VNTR. In conclusion, Haarlem 3 genotype was the predominant genotype among the isolates from MDR-TB cases in this study, which could be of special concern.
本研究旨在进一步了解伊朗首都德黑兰流行的耐多药（MDR）和广泛耐药（XDR）结核分枝杆菌分离株的遗传多样性。2010 年 1 月至 2015 年 3 月，从肺结核（TB）患者中分离出 723 株结核分枝杆菌。对 23 株 MDR、前 XDR 和 XDR 结核分枝杆菌分离株进行 spoligotyping 和 24 位位基因间重复单位可变数量串联重复（MIRU-VNTR）分型。结果表明，MDR、前 XDR 和 XDR 结核分枝杆菌菌株主要属于 Haarlem 3 基因型（11/23；47.8%），其次是北京家族（9/23；39.1%）。此外，23 株菌株使用 24 位 MIRU-VNTR 聚类为 21 种基因型。总之，Haarlem 3 基因型是本研究中 MDR-TB 病例分离株中的主要基因型，值得特别关注。
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