Department of Microbiology & Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA.
Department of Clinic Diagnosis, Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Microbes Infect. 2016 Nov;18(11):669-674. doi: 10.1016/j.micinf.2016.07.002. Epub 2016 Jul 18.
Ascending infection by sexually transmitted Chlamydia trachomatis is required for chlamydial induction of tubal pathology. To achieve ascension, the C. trachomatis organisms may have to spread from cell to cell, which inevitably exposes the organisms to extracellular mucosal effectors such as complement factors that are known to possess strong antichlamydial activities. Here, we report that the chlamydia-secreted protease CPAF efficiently neutralized complement factor C3-dependent antichlamydial activity. The neutralization was dependent on the proteolytic activity of CPAF and correlated with the CPAF-mediated degradation of complement factor C3 and factor B. As a result, CPAF preferentially inhibited the alternative complement activation pathway. The significance and limitation of these observations were discussed.
性传播沙眼衣原体的上行感染是沙眼衣原体诱导输卵管病变所必需的。为了实现上行感染，沙眼衣原体病原体可能不得不从一个细胞传播到另一个细胞，这不可避免地使病原体暴露于细胞外黏膜效应物中，如补体因子，已知这些因子具有很强的抗衣原体活性。在这里，我们报告衣原体分泌的蛋白酶 CP AF 可以有效地中和补体因子 C3 依赖的抗衣原体活性。这种中和作用依赖于 CP AF 的蛋白水解活性，并与 CP AF 介导的补体因子 C3 和因子 B 的降解相关。因此，CP AF 优先抑制替代补体激活途径。讨论了这些观察结果的意义和局限性。
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