Jensen T, Deckert M, Dawnay A, Feldt-Rasmussen B
Steno Memorial Hospital, Gentofte, Denmark.
Diabetes Res. 1989 Feb;10(2):93-5.
A highly sensitive enzyme linked immunoadsorbent assay for determining retinol-binding protein in urine and serum is described. Commercially available reagents are used. The standard curve ranges from 1.2-24 micrograms/l, recovery of retinol-binding protein added to urine was 99-102% (n = 10) and dilution of urine was linear. The within-assay coefficient of variation ranged from 1.2-3.1% and the day-to-day coefficient of variation from 9.2-10.5% depending on concentration. The correlation with urinary retinol-binding protein determined by radioimmunoassay was good (n = 90, r = 0.95). In vitro experiments show that retinol-binding protein is stable in urine with pH 5.2.
本文描述了一种用于测定尿液和血清中视黄醇结合蛋白的高灵敏度酶联免疫吸附测定法。使用市售试剂。标准曲线范围为1.2 - 24微克/升，添加到尿液中的视黄醇结合蛋白回收率为99 - 102%（n = 10），尿液稀释呈线性。根据浓度不同，批内变异系数范围为1.2 - 3.1%，日间变异系数范围为9.2 - 10.5%。与放射免疫测定法测定的尿视黄醇结合蛋白的相关性良好（n = 90，r = 0.95）。体外实验表明，视黄醇结合蛋白在pH 5.2的尿液中稳定。
