Głowacki Rafał, Furmaniak Paulina, Kubalczyk Paweł, Borowczyk Kamila
Department of Environmental Chemistry, Faculty of Chemistry, University of Łódź, 163 Pomorska Street, 90-236 Łódź, Poland.
J Anal Methods Chem. 2016;2016:3827832. doi: 10.1155/2016/3827832. Epub 2016 Jun 29.
Apigenin is a naturally occurring plant flavone that exhibits strong antioxidant, anti-inflammatory, and antitumor properties. A MEKC-UV based method was developed for the determination of total apigenin in selected herbs. Application of pseudostationary phase in the form of SDS micelles resulted in great repeatability of retention times and peak areas. A buffer solution consisting of 30 mmol/L sodium borate (pH 10.2), 10% acetonitrile, and 10 mmol/L sodium dodecyl sulfate was found to be the most suitable BGE for the separation. The method was validated and calibrated for total apigenin in the range of 1.0-100 μmol/L (R (2) = 0.9994). The limits of detection and quantification were 0.48 μmol/L and 0.92 μmol/L, respectively. This precise and robust method was successfully applied to the analysis of plant samples for total apigenin content.
芹菜素是一种天然存在的植物黄酮，具有强大的抗氧化、抗炎和抗肿瘤特性。开发了一种基于胶束电动毛细管色谱-紫外检测法来测定选定草药中的总芹菜素含量。以十二烷基硫酸钠胶束形式的假固定相的应用使得保留时间和峰面积具有很高的重复性。发现由30 mmol/L硼酸钠（pH 10.2）、10%乙腈和10 mmol/L十二烷基硫酸钠组成的缓冲溶液是最适合分离的背景电解质。该方法在1.0 - 100 μmol/L范围内对总芹菜素进行了验证和校准（R (2) = 0.9994）。检测限和定量限分别为0.48 μmol/L和0.92 μmol/L。这种精确且稳健的方法成功应用于植物样品总芹菜素含量的分析。
